Rootstocks Overexpressing StNPR1 and StDREB1 Improve Osmotic Stress Tolerance of Wild-Type Scion in Transgrafted Tobacco Plants
| Autor: | Praveen K. Saxena, Yasmine S. Hezema, Murali M. Ayyanath, Sherif M. Sherif, Alok Goel, Mukund R. Shukla |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine StNPR1 Osmotic shock QH301-705.5 Nicotiana tabacum Transgene Genetically modified crops 01 natural sciences Catalysis ORGs Inorganic Chemistry 03 medical and health sciences mRNA transport MRNA transport Physical and Theoretical Chemistry transgrafting Biology (General) Molecular Biology QD1-999 Spectroscopy biology Organic Chemistry fungi Wild type food and beverages ROS General Medicine biology.organism_classification StDREB1 Computer Science Applications Horticulture Chemistry 030104 developmental biology ABA osmotic stress Solanum Rootstock 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 8398, p 8398 (2021) International Journal of Molecular Sciences Volume 22 Issue 16 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | In grafted plants, the movement of long-distance signals from rootstocks can modulate the development and function of the scion. To understand the mechanisms by which tolerant rootstocks improve scion responses to osmotic stress (OS) conditions, mRNA transport of osmotic responsive genes (ORGs) was evaluated in a tomato/potato heterograft system. In this system, Solanum tuberosum was used as a rootstock and Solanum lycopersicum as a scion. We detected changes in the gene expression levels of 13 out of the 21 ORGs tested in the osmotically stressed plants of these, only NPR1 transcripts were transported across the graft union under both normal and OS conditions. Importantly, OS increased the abundance of StNPR1 transcripts in the tomato scion. To examine mRNA mobility in transgrafted plants, StNPR1 and StDREB1 genes representing the mobile and non-mobile transcripts, respectively, were overexpressed in tobacco (Nicotiana tabacum). The evaluation of transgenic tobacco plants indicated that overexpression of these genes enhanced the growth and improved the physiological status of transgenic plants growing under OS conditions induced by NaCl, mannitol and polyethylene glycol (PEG). We also found that transgenic tobacco rootstocks increased the OS tolerance of the WT-scion. Indeed, WT scions on transgenic rootstocks had higher ORGs transcript levels than their counterparts on non-transgenic rootstocks. However, neither StNPR1 nor StDREB1 transcripts were transported from the transgenic rootstock to the wild-type (WT) tobacco scion, suggesting that other long-distance signals downstream these transgenes could have moved across the graft union leading to OS tolerance. Overall, our results signify the importance of StNPR1 and StDREB1 as two anticipated candidates for the development of stress-resilient crops through transgrafting technology. |
| Databáze: | OpenAIRE |
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