Cytosolic Transport of Nanoparticles through Pressurized Plasma Membranes for Molecular Delivery and Amplification of Intracellular Fluorescence
| Autor: | Hidenobu Nakao, Tomoto Ura, Ling Chao, Shao-Wei Lyu, Joel Henzie, Yoshihisa Kaizuka |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
media_common.quotation_subject Endocytic cycle Metal Nanoparticles Nanoparticle Nanotechnology 02 engineering and technology Fluorescence 03 medical and health sciences Cytosol Electrochemistry Humans General Materials Science Cytosolic transport Internalization Spectroscopy media_common Chemistry Cell Membrane Biological Transport Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics HEK293 Cells 030104 developmental biology Colloidal gold Drug delivery Biophysics Gold 0210 nano-technology Intracellular HeLa Cells |
| Zdroj: | Langmuir. 32:13534-13545 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/acs.langmuir.6b03412 |
| Popis: | Transporting nanoparticles into live cells is important for drug delivery and other related applications. We found that cells exposed to hypoosmotic pressures can internalize substantial quantities of gold nanoparticles. Importantly, these nanoparticles can circumvent normal intracellular traffic and be transported directly into the cytosol, without the need for surface functionalization. In contrast, nanoparticles endocytosed at physiological osmolality are segregated inside endocytic organelles and are not able to reach the cytosol. Cytosolic internalization was observed for nanoparticles of various sizes and materials, with minimal short- or long-term damage induced by the internalized particles. Thus, our strategy can be used as a delivery platform for a range of applications from therapeutics to medical imaging. As examples, we demonstrated rapid delivery of membrane-impermeable molecules to the cytosol by using nanoparticles as carriers and the use of nanoparticles assembled within the cytosol as plasmonic nanoantenna to enhance intracellular fluorescence. We propose a model for the mechanisms behind nanoparticle internalization through pressurized plasma membranes via the release of lateral pressures. Such characterizations may constitute a foundation for developing new technologies, including nanoparticle-based drug delivery. |
| Databáze: | OpenAIRE |
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