Temperature sensitive SMA-causing point mutations lead to SMN instability, locomotor defects, and premature lethality inDrosophila
| Autor: | Suhana S. Singh, Ashlyn M. Spring, Amanda C. Raimer, Maina R. Edula, Tamara Paris-Davila, Vasudha Vandadi, A. Gregory Matera |
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| Rok vydání: | 2019 |
| Předmět: |
Mutation
Tudor domain animal diseases Point mutation Period (gene) Neuroscience (miscellaneous) Medicine (miscellaneous) Spinal muscular atrophy SMN1 Biology medicine.disease SMA medicine.disease_cause Phenotype General Biochemistry Genetics and Molecular Biology nervous system diseases Cell biology Immunology and Microbiology (miscellaneous) nervous system medicine Missense mutation |
| Popis: | Spinal muscular atrophy (SMA) is the leading genetic cause of death in young children, arising from homozygous deletion or mutation of theSMN1gene. SMN protein expressed from a paralogous gene,SMN2, is the primary genetic modifier of SMA; small changes in overall SMN levels cause dramatic changes in disease severity. Thus, deeper insight into mechanisms that regulate SMN protein stability should lead to better therapeutic outcomes. Here, we show that SMA patient-derived missense mutations in theDrosophilaSMN Tudor domain exhibit a pronounced temperature sensitivity that affects organismal viability, larval locomotor function, and adult longevity. These disease-related phenotypes are domain-specific and result from decreased SMN stability at elevated temperature. This system was utilized to manipulate SMN levels during various stages ofDrosophiladevelopment. Due to a large maternal contribution of mRNA and protein,Smnis not expressed zygotically during embryogenesis. Interestingly, we find that only baseline levels of SMN are required during larval stages, whereas high levels of protein are required during pupation. This previously uncharacterized period of elevated SMN expression, during which the majority of adult tissues are formed and differentiated, could be an important and translationally relevant developmental stage in which to study SMN function. Altogether, these findings illustrate a novelin vivorole for the SMN Tudor domain in maintaining SMN homeostasis and highlight the necessity for high SMN levels at critical developmental timepoints that is conserved fromDrosophilato humans. |
| Databáze: | OpenAIRE |
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