Small interfering RNAs inhibit infectious bursal disease virus replication in Vero cells
| Autor: | Chhabi Lal Patel, Anamika Mishra, B. B. Dash, Sonawane A, Saxena A, Ashwin Ashok Raut, Basavaraj Sajjanar, Alok Kumar Rai |
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| Rok vydání: | 2011 |
| Předmět: |
Small interfering RNA
animal structures Genetic Vectors Biology Virus Replication Infectious bursal disease virus Virus Infectious bursal disease Small hairpin RNA Viral Proteins RNA interference Virology Chlorocebus aethiops medicine Animals RNA Small Interfering Vero Cells Cells Cultured General Medicine medicine.disease In vitro Infectious Diseases Viral replication Vero cell RNA Interference |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1336-2305 |
| DOI: | 10.4149/av_2011_01_75 |
| Popis: | Small interfering RNA (siRNA) molecules are considered to be a promising antiviral therapeutics. This study was performed to analyze the application of siRNA against infectious bursal disease virus (IBDV) replication. Two siRNAs were designed to target common coding sequences of four IBDV proteins. Corresponding vectors were constructed to express anti-IBDV short hairpin RNAs (shRNA) that were tested for their antiviral effect in Vero cells. The results showed that expressed shRNA inhibited the virus replication to a significant extent (92%) as determined by the virus titration in cell culture. This outcome demonstrated the effectiveness of RNA interference (RNAi) based mechanism against the IBDV in vitro. |
| Databáze: | OpenAIRE |
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