Dependence of adaptive response and its bystander transmission on the genetic background of tested cells

Autor:	Lihua Zhang, George Iliakis, Holger Klammer, Munira Kadhim
Rok vydání:	2012
Předmět:	DNA End-Joining Repair Cell Survival Medizin Priming (immunology) Biology Radiation Tolerance Cell Line Ionizing radiation Mice Genes Reporter Radioresistance Bystander effect Animals Humans Radiology Nuclear Medicine and imaging Homologous Recombination A549 cell Radiological and Ultrasound Technology Bystander Effect Cell Cycle Checkpoints Fibroblasts Molecular biology Cell biology Non-homologous end joining Luminescent Proteins Cell culture embryonic structures Homologous recombination
Zdroj:	International Journal of Radiation Biology. 88:720-726
ISSN:	1362-3095 0955-3002
DOI:	10.3109/09553002.2012.691613
Popis:	Radiation-induced adaptive response (AR) is a phenomenon of increased radioresistance mediated by a low priming dose of ionizing radiation (IR) applied prior to a higher challenging dose. We have previously shown that in mouse-embryo fibroblasts (MEF) and human A549 cells, AR is associated with enhanced repair of DNA double-strand breaks (DSB) by the DNA-PK-dependent pathway of non-homologous end-joining (D-NHEJ). Importantly, AR was 'transmitted' to non-irradiated bystander cells through transfer of medium from cells that had received a priming dose of IR. Here, we examine the influence of the genetic background in these responses.Two plasmid-based assays specifically designed to measure the efficiency of NHEJ and HRR (homologous recombination repair) were deployed. MEF and the primary human fibroblast cell lines HF12 and HF19 were exposed to 10 mGy to 5 Gy X-rays. Bystander effects were investigated using the medium-transfer technique.In contrast to MEF, which induce robust AR to NHEJ, even as a bystander response, human fibroblasts fail to develop such phenomena.The development of AR is cell-type-specific. The same holds true for the development of AR as a bystander effect. Better understanding of the underlying mechanisms will help to understand the molecular basis of these differences in response.
Databáze:	OpenAIRE
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