The acute effect of intravenous lipopolysaccharide injection on serum and intrafollicular HDL components and gene expression in granulosa cells of the bovine dominant follicle
Autor: | Luiz Francisco Machado Pfeifer, Augusto Schneider, Diego Andres Velasco Acosta, Carlos Castilho Barros, Felipe Terres de Campos, Marcio Nunes Corrêa, Jorgea Pradieé, Pedro Augusto Silva Silveira, Joao Aveiro Alvarado Rincon, Ligia Margareth Cantareli Pegoraro, Bernardo Garziera Gasperin |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Lipopolysaccharides medicine.medical_specialty Lipopolysaccharide Granulosa cell Inflammation Biology 03 medical and health sciences chemistry.chemical_compound Food Animals Ovarian Follicle Internal medicine medicine Animals Ovarian follicle Small Animals Granulosa Cells Equine Aryldialkylphosphatase Follicular fluid Follicular Fluid 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Gene Expression Regulation TLR4 lipids (amino acids peptides and proteins) Animal Science and Zoology Tumor necrosis factor alpha Administration Intravenous Cattle Female medicine.symptom Estrus Synchronization Lipoproteins HDL Lipoprotein |
Zdroj: | Theriogenology. 89 |
ISSN: | 1879-3231 |
Popis: | The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) - estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P 0.05). The FF E2 and P4 concentrations were not different between groups (P 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells. |
Databáze: | OpenAIRE |
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