New mRNA probes for hippocampal responses to entorhinal cortex lesions in the adult male rat: a preliminary report
| Autor: | Heinz H. Osterburg, Nicholas J. Laping, Caleb E. Finch, Bon Hong Min, Jonathan R. Day, Glover Martin |
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| Rok vydání: | 1992 |
| Předmět: |
Male
endocrine system Pathology medicine.medical_specialty Synaptosomal-Associated Protein 25 Molecular Sequence Data Hippocampus Vimentin Nerve Tissue Proteins Hippocampal formation Functional Laterality Lesion Mice Developmental Neuroscience Sequence Homology Nucleic Acid medicine Animals RNA Messenger Cloning Molecular Gene Library Messenger RNA biology Base Sequence cDNA library Membrane Proteins RNA Probes Entorhinal cortex Molecular biology Rats Inbred F344 Rats nervous system Neurology Suppression subtractive hybridization Ferritins biology.protein medicine.symptom Plasmids |
| Zdroj: | Experimental neurology. 117(1) |
| ISSN: | 0014-4886 |
| Popis: | Three new mRNA responses were found in the hippocampus of the adult male rat after entorhinal cortex lesions (ECL) that induce synaptic reorganization. Hippocampus cDNA libraries were screened by a subtractive hybridization strategy designed to detect ECL-induced mRNAs. Partial sequencing showed clones with similarities to mouse vimentin, ferritin, and polypeptide 7B-2. A sequence similar to mouse SNAP-25 sequence was also detected. Using a mouse SNAP-25 probe, rat SNAP-25 mRNA increased in the hippocampus after ECL. |
| Databáze: | OpenAIRE |
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