Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella

Autor:	David R. Hendrixson, Daisuke Nakane, Yoshiki Kabata, Takayuki Nishizaka, Eli J. Cohen, Morgan Beeby
Přispěvatelé:	National Institutes of Health, Medical Research Council (MRC)
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Physiology PATHOGENESIS Video microscopy Pathology and Laboratory Medicine Biochemistry Physical Chemistry COLONIZATION Protein filament 1108 Medical Microbiology Materials Physics Microbial Physiology Flagellar Rotation Medicine and Health Sciences Bacterial Physiology Biology (General) HOOK 0303 health sciences biology Viscosity Physics CHEMOTAXIS Enterobacteriaceae Cell biology Bacterial Pathogens Cell Motility Chemistry 1107 Immunology Flagella Medical Microbiology Physical Sciences Cell Swimming Cellular Structures and Organelles Pathogens Life Sciences & Biomedicine 0605 Microbiology Research Article Pathogen Motility Virulence Factors QH301-705.5 Immunology Materials Science Motility Flagellum Campylobacter jejuni Microbiology 03 medical and health sciences MOTILITY Virology Genetics Molecular Biology Microbial Pathogens Swimming 030304 developmental biology Science & Technology Cell swimming Bacteria 030306 microbiology Biological Locomotion Organisms Biology and Life Sciences Proteins Bacteriology Campylobacter Cell Biology Flagellar Motility RC581-607 biology.organism_classification Chemical Properties ROTATION biology.protein Parasitology Immunologic diseases. Allergy Flagellin
Zdroj:	PLoS Pathogens, Vol 16, Iss 7, p e1008620 (2020) PLoS Pathogens
ISSN:	1553-7374 1553-7366
Popis:	Campylobacter jejuni rotates a flagellum at each pole to swim through the viscous mucosa of its hosts’ gastrointestinal tracts. Despite their importance for host colonization, however, how C. jejuni coordinates rotation of these two opposing flagella is unclear. As well as their polar placement, C. jejuni’s flagella deviate from the norm of Enterobacteriaceae in other ways: their flagellar motors produce much higher torque and their flagellar filament is made of two different zones of two different flagellins. To understand how C. jejuni’s opposed motors coordinate, and what contribution these factors play in C. jejuni motility, we developed strains with flagella that could be fluorescently labeled, and observed them by high-speed video microscopy. We found that C. jejuni coordinates its dual flagella by wrapping the leading filament around the cell body during swimming in high-viscosity media and that its differentiated flagellar filament and helical body have evolved to facilitate this wrapped-mode swimming. Author summary Campylobacter jejuni is a leading cause of gastroenteritis worldwide. This species uses its helical body and opposing flagella to drill its way through the viscous mucosa of host organisms’ gastrointestinal tracts. In this work, we show that C. jejuni coordinates its two opposing flagella by wrapping the leading flagellum around the cell body when swimming in viscous environments. We also provide evidence that the helical cell body of C. jejuni and its composite flagellar filament are important for wrapping and unwrapping of the flagellar filament during reversals of swimming direction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9efd76d1ef4eadb8f9319491ebc94dbd https://doaj.org/article/53677a8078504980a384cf3253fb6310 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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