The human papillomavirus 16 European-T350G E6 variant can immortalize but not transform keratinocytes in the absence of E7
| Autor: | Robert L. Jackson, Ingeborg Zehbe, Sarah Niccoli, Christina Richard, Melissa Togtema |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Keratinocytes
Human papillomavirus Genotype Papillomavirus E7 Proteins Population Viral Oncogene Gene Expression Oncogenicity Biology E6 protein Immunophenotyping Transformation 03 medical and health sciences 0302 clinical medicine Transduction Genetic Virology Humans education Cell Line Transformed Cell Proliferation 030304 developmental biology HPV16 variant Human papillomavirus 16 0303 health sciences education.field_of_study Cadherin Genetic Variation E-cadherin Oncogene Proteins Viral Cadherins Molecular biology Phenotype 3. Good health Repressor Proteins 030220 oncology & carcinogenesis E-T350G Biomarkers Immortalization |
| Zdroj: | Virology. 485:274-282 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2015.07.025 |
| Popis: | Human papillomavirus type 16 is commonly implicated in HPV-related cancers. However, only a small number of infected individuals progress to this stage. Epidemiological evidence demonstrated that oncogenic risk is population-specific and variations within the viral oncogene, E6, have been suggested to play a role in these findings. Of focus in this study is the European-T350G variant, which is characterized by an L>V amino acid substitution at residue 83 of the prototype E6 protein. To elucidate the functional effects of this polymorphism, we followed keratinocytes transduced with E-T350G E6 for over 60 passages and compared them to keratinocytes transduced, in parallel, with prototype or Asian-American (Q14H/L83V/H78Y) E6. We found that although E-T350G E6 immortalized transduced keratinocytes in the absence of E7, these cells were not fully transformed. We also found that E-T350G down-regulated E-cadherin compared to the other variants, providing a possible link between its population-based oncogenicity and host genetic variations. |
| Databáze: | OpenAIRE |
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