Molecular determination of van genes among clinical isolates of enterococci at a hospital setting
| Autor: | Harish Kumar Ks, Ahmed Adlan Mohammed Adam, Yaser A Al-Naam, Elmoeiz A Elnagi, Siju K. George, Saleh El Safi, Thekra N Al-Maqati, Ashly Mary Jacob, M. R. Suseela |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine medicine.drug_class QH301-705.5 Antibiotics Tigecycline medicine.disease_cause Antimicrobial resistance 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance Multiplex polymerase chain reaction medicine Enterococcus casseliflavus Biology (General) biology biochemical phenomena metabolism and nutrition Multiplex PCR biology.organism_classification bacterial infections and mycoses 030104 developmental biology chemistry Staphylococcus aureus Vancomycin-resistant enterococci Linezolid General Agricultural and Biological Sciences 010606 plant biology & botany Enterococcus faecium medicine.drug |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 28, Iss 5, Pp 2895-2899 (2021) |
| Popis: | Vancomycin-resistant enterococci (VRE) poses a formidable challenge to public health due to its inherent resistance to multiple antibiotics coupled with the ability to transfer genetic determinants to dangerous pathogens like Methicillin-resistant Staphylococcus aureus (MRSA). The purpose of this study was to investigate the incidence of vancomycin resistance in enterococci among clinical isolates at a tertiary care military hospital in the eastern region of Saudi Arabia and to detect van genes using multiplex-PCR. Overall, 246 isolates of enterococci were collected from various clinical specimens. The isolates were identified, and antimicrobial susceptibility testing was done using the Vitek 2 system. Multiplex PCR was performed on the VRE isolates, thus identified to determine the van genes harbored. A total of 15 VRE were identified, of which 14 (93.3%) were Enterococcus faecium, and 1(6.7%) was Enterococcus casseliflavus with intrinsic vanC resistance. Of the 14 vancomycin-resistant Enterococcus faecium, 8 (57.1%) harbored vanB genes, while 6 (42.8%) harbored vanA genes. All the VRE were susceptible to linezolid and tigecycline. Our study detected a low prevalence (6.1%) of VRE among clinical isolates of enterococci and that the vanB gene predominates in such strains. Susceptibility profiles indicated that linezolid and tigecycline are still effective against these multidrug-resistant pathogens. Pus specimens yielded the highest percentage (53.3%) of isolates from which VRE was obtained, and this finding is novel among studies done in Saudi Arabia. |
| Databáze: | OpenAIRE |
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