Phylogenetic analysis of human rhinoviruses collected over four successive years in Sydney, Australia

Autor: Frank Zeng, Dominic E. Dwyer, Vigneswary Mala Ratnamohan, Chandini Raina MacIntyre, Jen Kok, Linda Donovan
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Time Factors
Rhinovirus
Epidemiology
viruses
Subspecies
phylogeny
law.invention
law
Nasopharynx
Genotype
Prevalence
Child
Respiratory Tract Infections
Polymerase chain reaction
influenza‐like illness
Phylogenetic tree
virus diseases
Respiratory infection
Infectious Diseases
medicine.anatomical_structure
RNA
Viral
Female
Original Article
circulatory and respiratory physiology
Adult
Pulmonary and Respiratory Medicine
030106 microbiology
Nose
Biology
Real-Time Polymerase Chain Reaction
03 medical and health sciences
stomatognathic system
Phylogenetics
Throat
otorhinolaryngologic diseases
medicine
Humans
human rhinovirus
Influenza-like illness
Picornaviridae Infections
molecular typing
Australia
Public Health
Environmental and Occupational Health
Genetic Variation
Sequence Analysis
DNA
Original Articles
Virology
030104 developmental biology
Pharynx
Zdroj: Influenza and Other Respiratory Viruses
ISSN: 1750-2659
1750-2640
Popis: Background Human rhinoviruses (HRV) cause a wide spectrum of disease, ranging from a mild influenza-like illness (ILI) to severe respiratory infection. Molecular epidemiological data are limited for HRV circulating in the Southern Hemisphere. Objectives To identify the species and genotypes of HRV from clinical samples collected in Sydney, Australia, from 2006 to 2009. Methods Combined nose and throat swabs or nasopharyngeal aspirates collected from individuals with ILI were tested for HRV using real-time reverse-transcriptase polymerase chain reaction (RT-PCR). Sequencing data of 5′UTR and VP4/VP2 coding regions on RT-PCR-positive specimens were analysed. Results Human rhinoviruses were detected by real-time PCR in 20.9% (116/555) of samples tested. Phylogenetic analysis of 5′UTR and VP4/VP2 on HRV-positive samples was concordant in the grouping of HRV A and B species but not HRV C species. Eighty per cent (16/20) of sequences that grouped as HRV C in the VP4/VP2 tree clustered as HRV A, alongside some previously described C strains as subspecies C/A. Discordant branching was seen within HRV A group: two sequences clustering as A in the VP4/VP2 tree branched within the C/A subspecies in the 5′UTR tree, and one sequence showed identity to different HRV A strains in the two genes. The prevalence of HRV C and C/A species was greater in paediatric compared to adult patients (47.9% vs 25.5%, P = .032). Conclusion Human rhinoviruses are a common cause of respiratory infections, and HRV C is present in the Southern Hemisphere. Sequencing of multiple HRV regions may be necessary to determine exact phylogenetic relationships.
Databáze: OpenAIRE
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