Complement component C1q is produced by isolated articular chondrocytes
| Autor: | J.C. Kwekkeboom, R.A. van Schaarenburg, R. Mahdad, Rik Lories, Andreea Ioan-Facsinay, A Bakker, Silvia Monteagudo, Chahrazad Cherifi, R. Lubbers, Rem Toes, E W N Levarht, Leendert A. Trouw |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cartilage
Articular 0301 basic medicine Complement Biomedical Engineering Pilot Projects Chondrocyte Mice 03 medical and health sciences Chondrocytes 0302 clinical medicine Rheumatology Downregulation and upregulation Western blot Osteoarthritis medicine Extracellular Protein biosynthesis Animals Humans Orthopedics and Sports Medicine RNA Messenger Collagen Type II C1q 030203 arthritis & rheumatology Messenger RNA Complement C1s medicine.diagnostic_test Complement C1r Chemistry Complement C1q Cartilage Osteoarthritis Knee Complement system Cell biology 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Collagen Type X |
| Zdroj: | Osteoarthritis and Cartilage Osteoarthritis and Cartilage, 28(5), 675-684. ELSEVIER SCI LTD |
| ISSN: | 1063-4584 |
| Popis: | OBJECTIVE: Inflammation and innate immune responses may contribute to development and progression of Osteoarthritis (OA). Chondrocytes are the sole cell type of the articular cartilage and produce extracellular-matrix molecules. How inflammatory mediators reach chondrocytes is incompletely understood. Previous studies have shown that chondrocytes express mRNA encoding complement proteins such as C1q, suggesting local protein production, which has not been demonstrated conclusively. The aim of this study is to explore C1q production at the protein level by chondrocytes. DESIGN: We analysed protein expression of C1q in freshly isolated and cultured human articular chondrocytes using Western blot, ELISA and flow cytometry. We examined changes in mRNA expression of collagen, MMP-1 and various complement genes upon stimulation with pro-inflammatory cytokines or C1q. mRNA expression of C1 genes was determined in articular mouse chondrocytes. RESULTS: Primary human articular chondrocytes express genes encoding C1q, C1QA, C1QB, C1QC, and secrete C1q to the extracellular medium. Stimulation of chondrocytes with pro-inflammatory cytokines upregulated C1QA, C1QB, C1QC mRNA expression, although this was not confirmed at the protein level. Extracellular C1q bound to the chondrocyte surface dose dependently. In a pilot study, binding of C1q to chondrocytes resulted in changes in the expression of collagens with a decrease in collagen type 2 and an increase in type 10. Mouse articular chondrocytes also expressed C1QA, C1QB, C1QC, C1R and C1S at the mRNA level. CONCLUSIONS: C1q protein can be expressed and secreted by human articular chondrocytes and is able to bind to chondrocytes influencing the relative collagen expression. ispartof: OSTEOARTHRITIS AND CARTILAGE vol:28 issue:5 pages:675-684 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect