Feline Leukemia Virus p27 Antigen Concentration and Proviral DNA Load Are Associated with Survival in Naturally Infected Cats
| Autor: | Jesse Buch, Marko Estrada, Andrei Rakitin, Ellen P. Jefferson, E. Susan Amirian, Julie Levy, Melissa J. Beall, Natascha T. Hamman, Genevieve Clark, Monica K. Frenden |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
FeLV longitudinal 040301 veterinary sciences Gene Dosage lcsh:QR1-502 Proviral dna progressive Biology Feline leukemia virus survival Article lcsh:Microbiology law.invention 0403 veterinary science 03 medical and health sciences Antigen Proviruses quantitative law Virology diagnostics Animals Prospective Studies Prospective cohort study Antigens Viral Polymerase chain reaction CATS Receiver operating characteristic Leukemia Virus Feline Significant difference regressive 04 agricultural and veterinary sciences Viral Load biology.organism_classification 030104 developmental biology Infectious Diseases DNA Viral Leukemia Feline Cats Female Retroviridae Infections |
| Zdroj: | Viruses, Vol 13, Iss 302, p 302 (2021) Viruses Volume 13 Issue 2 |
| ISSN: | 1999-4915 |
| Popis: | Longitudinal studies of cats naturally infected with feline leukemia virus (FeLV) are important for understanding disease outcomes. Levels of p27 antigen and copy numbers of proviral DNA have been associated with FeLV-infection courses. The purpose of this prospective study was to establish cutoff values for p27 antigen concentration and proviral DNA load that distinguished high positive from low positive groups of cats and to evaluate an association with survival. At enrollment, 254 cats were tested by point-of-care and microtiter plate enzyme-linked immunosorbent assays (ELISAs) for p27 antigen and real-time polymerase chain reaction (PCR) for proviral DNA. The 127 positive cats were retested monthly for six months and monitored for survival over the four-year study. A receiver operating characteristic-based analysis of samples with concordant or discordant qualitative results for p27 antigen and proviral DNA was used to establish cutoff values, and when applied to test results at enrollment for classifying cats as high positive or low positive, a significant difference in survival was observed. High positive cats had a median survival of 1.37 years (95% CI 0.83–2.02) from time of enrollment, while most low positive cats were still alive (93.1% survival). Quantitative results for p27 antigen concentration and proviral DNA load were highly correlated with survival times in FeLV-infected cats. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|