Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
| Autor: | Mauricio G. C. Resende, R.B. Flatschart |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
polymerase chain reaction animal diseases viruses lcsh:QR1-502 Virulence Biology Herpesvirus suis Microbiology Virus lcsh:Microbiology law.invention law reação em cadeia da polimerase Gene Polymerase chain reaction Trigeminal nerve chemistry.chemical_classification Aujeszkys disease virus Strain (chemistry) vírus da doença de Aujeszky virus diseases hibridização dot-blot Virology chemistry Glycoprotein dot-blot hybridization |
| Zdroj: | Brazilian Journal of Microbiology, Vol 31, Iss 4, Pp 308-311 (2000) Brazilian Journal of Microbiology v.31 n.4 2000 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| ISSN: | 1678-4405 |
| Popis: | Acute and latent infections with the Brazilian LA031 strain of Aujeszkys disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization. |
| Databáze: | OpenAIRE |
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