Cardiorespiratory evaluation of juvenile rats experimentally envenomed with Tityus serrulatus venom
Autor: | M.C.L. Pinto, Marcos B. Melo, D. Verçosa Júnior, Marília Martins Melo, M. L. Cruz |
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Rok vydání: | 2010 |
Předmět: |
Pathology
medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine Tityus serrulatus Myocarditis lcsh:RC955-962 electrocardiography Venom Toxicology Subcutaneous injection lcsh:RA1190-1270 pulmonary hemorrhages lcsh:Zoology medicine lcsh:QL1-991 Envenomation lcsh:Toxicology. Poisons biology troponin business.industry biology.organism_classification medicine.disease Troponin rats CK-MB Infectious Diseases CK biology.protein Animal Science and Zoology Parasitology Creatine kinase Electrical conduction system of the heart business |
Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 16, Issue: 2, Pages: 253-267, Published: 2010 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 2, Pp 253-267 (2010) Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.2 2010 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP |
ISSN: | 1678-9199 |
Popis: | Accidental envenomation caused by Tityus serrulatus scorpions is very common in Brazil and may result in serious cardiorespiratory alterations that are frequently fatal to children. In the present study, the effects of T. serrulatus venom on the cardiorespiratory system of recently weaned male Wistar rats were evaluated. Fifteen animals were distributed into three groups (n = 5). The control group A received 400 μL ultrapure water by subcutaneous injection, while the experimental groups B and C were injected with scorpion venom (100 and 450 μg, respectively, in 400 μL water). Electrocardiogram (ECG) traces were obtained prior to the experiment, at five-minute intervals up to 30 minutes after treatment. At 40 minutes after envenomation, the animals had severe acute symptoms and were subsequently anesthetized for blood collection by means of intracardiac puncture. Biochemical profiles for the cardiac muscle were established by colorimetric analysis of creatine kinase (CK) and CK-MB isoenzyme. Semiquantitative analysis of troponin was performed using the immunochromatographic assay. Following euthanasia, the lungs and hearts were removed and subjected to histopathological examination. All experimental animals had ECG alterations compatible with electrolytic imbalance, myocarditis and alterations of the cardiac conduction system. Envenomed animals had accentuated bradycardia at 25 and 30 minutes after venom inoculation. All experimental animals had myocardial lesions, which were confirmed by increased serum levels of CK and CK-MB, although there were no alterations in the serum concentration of troponin. Pulmonary hemorrhage was detected in whole lungs and microscopically confirmed by the presence of congested capillaries and erythrocytes in the alveolar parenchyma. In conclusion, T. serrulatus venom caused great cardiorespiratory damage to weaned rats. |
Databáze: | OpenAIRE |
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