Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement
Autor: | Qiang Niu, Pei Li, Yan Ma, Min-Jun Wang, Li Chen, Hui Cheng, Yi-Ping Hu, Lin Zhuang, Ping Zhu, Xiaoxia Hu, Qian Wang, Changcheng Liu, Miao-Xia He, Hui Jiang, Xiaoping Xu |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Telomerase medicine.medical_treatment Science Cell Cell Culture Techniques Biology Article Immunophenotyping Mice 03 medical and health sciences 0302 clinical medicine Tumor Cells Cultured medicine Animals Humans Autocrine signalling Cell Proliferation Chromosome Aberrations Gene Rearrangement Multidisciplinary Cell growth Chromosomes Human Pair 11 Chromosome Histone-Lysine N-Methyltransferase Gene rearrangement Precursor Cell Lymphoblastic Leukemia-Lymphoma Molecular biology 030104 developmental biology medicine.anatomical_structure Cytokine 030220 oncology & carcinogenesis Medicine Myeloid-Lymphoid Leukemia Protein Neoplasm Transplantation |
Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | At present, all cell strains derived from acute lymphoblastic leukemia (ALL) patients with the long arm of chromosome 11 aberration are accompanied with mixed lineage leukemia (MLL) gene rearrangement. In this study, we established a permanent ALL cell strain CHH-1 with the long arm of chromosome 11 aberration and without MLL rearrangement, hoping that it could be used for the research of ALL with such genetic abnormality. CHH-1 cell strain was certified through morphology, immunophenotype, genetics and immunoglobulin (Ig) gene rearrangement analysis. Cell characteristics including tumorigenic ability, semisolid colony forming ability, telomerase activity, autocrine and invasion were further detected. Cells were with an add(11)(q23) structural abnormality without MLL rearrangement, and were consistent with the genetic abnormality of the patient. In addition, these cells had features of tumor-forming ability, high colony forming capacity, unique cytokine autocrine mode, high telomerase activity, and high invasion ability. CHH-1 may prove to be a useful cell model for the research of human leukemia with genetic aberration in chromosome 11, and help explore the role of such genetic abnormality in the pathogenesis, progression and prognosis of ALL, and in developing new target drugs. |
Databáze: | OpenAIRE |
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