Inhibitory Effects of Momordicine I on High-Glucose-Induced Cell Proliferation and Collagen Synthesis in Rat Cardiac Fibroblasts
| Autor: | Neng Lang Shih, Chun Chao Chen, Ju Chi Liu, Po-Yuan Chen, Wen Rui Hao, Li Chin Sung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aging Cell signaling Antioxidant Article Subject Cardiac fibrosis NF-E2-Related Factor 2 medicine.medical_treatment Smad Proteins SMAD 030204 cardiovascular system & hematology medicine.disease_cause Biochemistry Models Biological Antioxidants Rats Sprague-Dawley Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine medicine Animals Phosphorylation RNA Small Interfering lcsh:QH573-671 Fibroblast Cell Proliferation Quassins Cell growth Chemistry lcsh:Cytology Myocardium Cell Biology General Medicine Fibroblasts medicine.disease Cell biology Sterols 030104 developmental biology medicine.anatomical_structure Glucose Collagen Reactive Oxygen Species Corrigendum Oxidative stress Signal Transduction Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2018 (2018) |
| ISSN: | 1942-0994 1942-0900 |
| Popis: | Diabetes-associated cardiac fibrosis is a severe cardiovascular complication. Momordicine I, a bioactive triterpenoid isolated from bitter melon, has been demonstrated to have antidiabetic properties. This study investigated the effects of momordicine I on high-glucose-induced cardiac fibroblast activation. Rat cardiac fibroblasts were cultured in a high-glucose (25 mM) medium in the absence or presence of momordicine I, and the changes in collagen synthesis, transforming growth factor-β1 (TGF-β1) production, and related signaling molecules were assessed. Increased oxidative stress plays a critical role in the development of high-glucose-induced cardiac fibrosis; we further explored momordicine I’s antioxidant activity and its effect on fibroblasts. Our data revealed that a high-glucose condition promoted fibroblast proliferation and collagen synthesis and these effects were abolished by momordicine I (0.3 and 1 μM) pretreatment. Furthermore, the inhibitory effect of momordicine I on high-glucose-induced fibroblast activation may be associated with its activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the inhibition of reactive oxygen species formation, TGF-β1 production, and Smad2/3 phosphorylation. The addition of brusatol (a selective inhibitor of Nrf2) or Nrf2 siRNA significantly abolished the inhibitory effect of momordicine I on fibroblast activation. Our findings revealed that the antifibrotic effect of momordicine I was mediated, at least partially, by the inhibition of the TGF-β1/Smad pathway, fibroblast proliferation, and collagen synthesis through Nrf2 activation. Thus, this work provides crucial insights into the molecular pathways for the clinical application of momordicine I for treating diabetes-associated cardiac fibrosis. |
| Databáze: | OpenAIRE |
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