Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy
| Autor: | Cody Reeves, Vasil N. Georgiev, Petia M. Vlahovska, Rumiana Dimova, Hammad A. Faizi |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Flexural modulus Vesicle Bilayer Confocal FOS: Physical sciences Flexural rigidity 02 engineering and technology General Chemistry Condensed Matter - Soft Condensed Matter 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences law.invention Membrane Biological Physics (physics.bio-ph) Confocal microscopy law Biophysics Soft Condensed Matter (cond-mat.soft) Physics - Biological Physics 0210 nano-technology Spectroscopy |
| Zdroj: | Soft Matter |
| ISSN: | 1744-6848 1744-683X |
| DOI: | 10.1039/d0sm00943a |
| Popis: | A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies. |
| Databáze: | OpenAIRE |
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