Live cell lithography: using optical tweezers to create synthetic tissue
| Autor: | Paul Matsudaira, Gregory Timp, Jan Scrimgeour, Kaethe Beck, Winston Timp, Mustafa Mir, Utkur Mirsaidov |
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| Rok vydání: | 2008 |
| Předmět: |
Escherichia
Materials science Optical Tweezers Genetically engineered Surface Properties Reference array Microfluidics Biomedical Engineering Bioengineering Nanotechnology General Chemistry Matrix (biology) Biochemistry Fluorescence Hydrogel Polyethylene Glycol Dimethacrylate Optical tweezers Biomimetic Materials Metabolic activity Lithography |
| Zdroj: | Lab on a chip. 8(12) |
| ISSN: | 1473-0197 |
| Popis: | We demonstrate a new method for creating synthetic tissue that has the potential to capture the three-dimensional (3D) complexity of a multi-cellular organism with submicron precision. Using multiple laminar fluid flows in a microfluidic network, we convey cells to an assembly area where multiple, time-shared optical tweezers are used to organize them into a complex array. The cells are then encapsulated in a 30 microm x 30 microm x 45 microm volume of photopolymerizable hydrogel that mimicks an extra-cellular matrix. To extend the size, shape and constituency of the array without loss of viability, we then step to an adjacent location while maintaining registration with the reference array, and repeat the process. Using this step-and-repeat method, we formed a heterogeneous array of E. coli genetically engineered with a lac switch that is functionally linked to fluorescence reporters. We then induced the array using ligands through a microfluidic network and followed the space-time development of the fluorescence to evaluate viability and metabolic activity. |
| Databáze: | OpenAIRE |
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