Comparative Efficacy of Topical Versus Systemic Teicoplanin in Experimental Model of Wound Infections
| Autor: | Oriana Simonetti, Annamaria Offidani, Aldo Bertani, Oscar Cirioni, Federico Mocchegiani, Gaia Goteri, Andrea Giacometti, Agnese Della Vittoria, Fiorenza Orlando, Carmela Silvestri, Vittorio Saba, Alessandra Filosa, Alessandro Scalise, Roberto Ghiselli, Giorgio Scalise |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Pathology Micrococcaceae medicine.drug_class Administration Topical Antibiotics medicine.disease_cause Gastroenterology Mice chemistry.chemical_compound Internal medicine medicine Animals Surgical Wound Infection Skin Antibacterial agent Mice Inbred BALB C biology Teicoplanin business.industry Microcirculation Granulation tissue Staphylococcal Infections biology.organism_classification Anti-Bacterial Agents Vascular endothelial growth factor Disease Models Animal medicine.anatomical_structure chemistry Staphylococcus aureus Injections Intravenous Methicillin Resistance Surgery business Injections Intraperitoneal medicine.drug Subcutaneous tissue |
| Zdroj: | Journal of Surgical Research. 144:74-81 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2007.02.051 |
| Popis: | Introduction Surgical site infections are the second most common cause of nosocomial infections and, typically, gram-positive pathogens are involved. Materials and methods A mouse model was used to investigate the efficacy of different methods for the treatment of wound infections. A full thickness wound was established on the back subcutaneous tissue of adult male BALB/c mice. A small gauze was placed over each wound and then inoculated with 5 × 10 7 colony-forming units of Staphylococcus aureus . The study included a control group that did not receive any treatment and four contaminated groups treated, respectively, with: (1) drug-free Allevyn (Smith and Nephew Healthcare, Yorkshire, United Kingdom), (2) teicoplanin-soaked Allevyn, (3) drug-free Allevyn and daily intraperitoneal teicoplanin (7 mg/kg) and, finally, (4) teicoplanin-soaked Allevyn and daily intraperitoneal teicoplanin (7 mg/kg). Main outcome measures were quantitative bacterial culture, assessment of vascular endothelial growth factor (VEGF) plasma levels, histological examination with assessment of microvessel density, and of VEGF expression in tissue sections. Results Data analysis showed that strong inhibition of bacterial growth was achieved in any group treated with intraperitoneal teicoplanin. However, the highest inhibition of bacterial growth was obtained in the group that received teicoplanin-soaked Allevyn and intraperitoneal teicoplanin. Histological examination showed that each treatment modality was able to reduce the delay in wound repair. The most effective treatment appeared to be the local application of teicoplanin-soaked hydro gel foam. The tissue effects were associated with an increase in neovascularization and VEGF expression by endothelial cells and fibroblasts in the granulation tissue. Bacterial colonies also were reduced, especially when teicoplanin was given parenterally. Conclusions Soaking a hydro cellular foam with an antistaphylococcal agents, such as teicoplanin, may be useful for the management of infected wounds. |
| Databáze: | OpenAIRE |
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