Coordinate expression of activating Fc gamma receptors I and III and inhibiting Fc gamma receptor type II in the determination of joint inflammation and cartilage destruction during immune complex-mediated arthritis
| Autor: | A.E.M. Holthuysen, K. C. A. M. Nabbe, P.L.E.M. van Lent, Sjef Verbeek, Johannes Roth, Andreas Blom, W.B. van den Berg, Peter Boross |
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| Rok vydání: | 2003 |
| Předmět: |
Tissue engineering and reconstructive surgery [UMCN 4.3]
Knee Joint Immunology Arthritis Gene Expression Inflammation Antigen-Antibody Complex Matrix metalloproteinase Severity of Illness Index Chondrocyte Immunophenotyping Mice Chondrocytes Rheumatology medicine Immunology and Allergy Animals Pharmacology (medical) Receptor Chronic inflammation and autoimmunity [UMCN 4.2] Mice Knockout Cell Death Chemistry Cartilage Macrophages Receptors IgG Immunotherapy gene therapy and transplantation [UMCN 1.4] medicine.disease Molecular biology Immune complex Mice Inbred C57BL medicine.anatomical_structure Fc-Gamma Receptor medicine.symptom |
| Zdroj: | Arthritis and Rheumatism, 48, 1, pp. 255-65 Arthritis and Rheumatism, 48, 255-65 |
| ISSN: | 0004-3591 |
| Popis: | Item does not contain fulltext OBJECTIVE: To study the role of the activating Fc gamma receptor types I and III (Fc gamma RI and Fc gamma RIII, respectively) and the inhibiting Fc gamma receptor II (Fc gamma RII) in inflammation and in various aspects of cartilage destruction during arthritis that is solely induced by immune complexes. METHODS: Immune complex-mediated arthritis (ICA) was passively induced by lysozyme-antilysozyme complexes in Fc gamma RI-, Fc gamma RIII-, and Fc gamma RII-knockout mice and their wild-type controls. Total knee joints were isolated to study inflammation and cartilage destruction (loss of proteoglycans [PGs], chondrocyte death, matrix metalloproteinase [MMP]-mediated neoepitope [VDIPEN] expression, and erosion). The presence of an active phenotype of macrophages was studied by detection of myeloid-related proteins 8 and 14 (MRP8 and MRP14, respectively). RESULTS: Influx and activation of inflammatory cells (MRP expression) during ICA was decreased in Fc gamma RIII-deficient mice and enhanced in mice lacking Fc gamma RII. Mild cartilage destruction reflected by loss of PGs was consistent with the degree of inflammation. Mice lacking Fc gamma RIII showed almost no PG depletion, whereas in Fc gamma RII(-/-) mice, PG depletion was increased 3-7-fold in various cartilage areas. Initiation of erosive cartilage destruction, as reflected by MMP-mediated VDIPEN expression, was reduced in Fc gamma RIII(-/-) and Fc gamma RI(-/-) mice, directing the two different critical steps of cellular influx and subsequent activation. These aspects were enhanced in Fc gamma RII(-/-) mice. In Fc gamma RI(-/-) and Fc gamma RIII(-/-) mice, VDIPEN expression was 90-99% lower, whereas in Fc gamma RII(-/-) mice, VDIPEN expression was increased 4-fold. Chondrocyte death was reduced in Fc gamma RIII(-/-) mice (68% lower) and enhanced in Fc gamma RII(-/-) mice (6-12-fold higher). Progression of arthritis and erosion of the cartilage surface were markedly elevated in Fc gamma RII(-/-) arthritic joints. CONCLUSION: During ICA, Fc gamma RIII is the dominant activating receptor mediating joint inflammation, whereas both Fc gamma RI and Fc gamma RIII are involved in cartilage destruction. Fc gamma RII inhibits both joint inflammation and severe cartilage destruction during ICA. |
| Databáze: | OpenAIRE |
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