Cyclic nucleotide-dependent protein kinases in the lower esophageal sphincter
| Autor: | H.S. Ormsbee, T.J. Torphy, C. A. Miller, M S Barnette |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
Physiology Guanosine Biology chemistry.chemical_compound Cyclic nucleotide Adenosine Triphosphate Dogs Esophagus Opossum Physiology (medical) Cyclic AMP otorhinolaryngologic diseases medicine Animals Humans Nucleotide Protein kinase A Cyclic GMP chemistry.chemical_classification Hepatology Kinase Isoproterenol Gastroenterology Opossums biology.organism_classification Adenosine Kinetics chemistry Biochemistry Female Protein Kinases Adenosine triphosphate medicine.drug |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 251:G794-G803 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.1986.251.6.g794 |
| Popis: | The characteristics and regulation of adenosine 3',5'-cyclic monophosphate (cAMP)-dependent and guanosine 3',5'-cyclic monophosphate (cGMP)-dependent protein kinases (PKs) in opossum, canine, and human lower esophageal sphincter (LES) were investigated. As measured by the incorporation of 32P from [gamma-32P]adenosine 5'-triphosphate (ATP) into histone, LES homogenates from all three species contained three distinct types of PK: cAMP-dependent PK, cGMP-dependent PK, and cyclic nucleotide-independent PK. In all three species, cAMP-dependent PK comprised approximately 80%, cGMP-dependent PK comprised approximately 10%, and cyclic nucleotide-independent PK comprised approximately 10% of the total PK activity in the LES. Diethylaminoethyl-sepharose column chromatography of the supernatant fraction of opossum LES homogenates revealed that of the total cAMP-dependent PK, 10% was Type I and 90% was Type II. In contrast, equal amounts of Type I and Type II cAMP-dependent PK were present in both the human and canine LES. Isoproterenol-induced relaxation of the isolated opossum LES was accompanied by an increase in cAMP content and an activation of cAMP-dependent PK. The results of this study support the proposal that cyclic nucleotides and cyclic nucleotide-dependent PKs regulate LES tone. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|