Induction of multi-antigen multi-stage immune responses against Plasmodium falciparum in rhesus monkeys, in the absence of antigen interference, with heterologous DNA prime/poxvirus boost immunization

Autor: Elizabeth Strobert, Walter R. Weiss, Harini Ganeshan, George Jiang, Nancy O. Richie, Ivette Caro-Aquilar, Yupin Charoenvit, Victoria Fallarme, Denise L. Doolan, Daniel J. Carucci, Noelle B. Patterson, Alberto Moreno, Maria F. Baraceros, Allan Saul, Laura B. Martin, David E. Lanar, Andrew J Geall, Kalpana Gowda, Mary R. Galinski, Craig Morrissette, David C. Kaslow, Steve Abot, Glenna Banania
Rok vydání: 2007
Předmět:
Zdroj: Malaria Journal, Vol 6, Iss 1, p 135 (2007)
Malaria Journal
ISSN: 1475-2875
Popis: The present study has evaluated the immunogenicity of single or multiplePlasmodium falciparum (Pf)antigens administered in a DNA prime/poxvirus boost regimen with or without the poloxamer CRL1005 in rhesus monkeys. Animals were primed withPfCSP plasmid DNA or a mixture ofPfCSP,PfSSP2/TRAP,PfLSA1,PfAMA1 andPfMSP1-42 (CSLAM) DNA vaccines in PBS or formulated with CRL1005, and subsequently boosted with ALVAC-Pf7, a canarypox virus expressing the CSLAM antigens. Cell-mediated immune responses were evaluated by IFN-γ ELIspot and intracellular cytokine staining, using recombinant proteins and overlapping synthetic peptides. Antigen-specific and parasite-specific antibody responses were evaluated by ELISA and IFAT, respectively. Immune responses to all components of the multi-antigen mixture were demonstrated following immunization with either DNA/PBS or DNA/CRL1005, and no antigen interference was observed in animals receiving CSLAM as compared toPfCSP alone. These data support the down-selection of the CSLAM antigen combination. CRL1005 formulation had no apparent effect on vaccine-induced T cell or antibody responses, either before or after viral boost. In high responder monkeys, CD4+IL-2+ responses were more predominant than CD8+ T cell responses. Furthermore, CD8+ IFN-γ responses were detected only in the presence of detectable CD4+ T cell responses. Overall, this study demonstrates the potential for multivalentPfvaccines based on rational antigen selection and combination, and suggests that further formulation development to increase the immunogenicity of DNA encoded antigens is warranted.
Databáze: OpenAIRE
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