Analysis of Products of theEscherichia coliGenomic Genes and Regulation of Their Expressions: An Applicable Procedure for Genomic Analysis of Other Microorganisms
| Autor: | Satoshi Yanai, Ali Azam Talukder, Mamoru Yamada |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
clone (Java method)
Molecular Sequence Data lac operon Biology medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Genome Analytical Chemistry chemistry.chemical_compound Bacterial Proteins Gene expression Escherichia coli medicine Genomic library Cloning Molecular Molecular Biology Gene DNA Primers Gene Library Genetics Base Sequence Organic Chemistry Gene Expression Regulation Bacterial General Medicine chemistry Genome Bacterial DNA Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 58:117-120 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.58.117 |
| Popis: | A partial library of the Escherichia coli genomic genes has been constructed, in which each clone has part of a genomic gene fused in frame with the lacZ gene in addition to its promoter and operator. DNA of randomly selected clones was sequenced, and the resultant deduced N-terminal amino acid sequences showed that 17 out of 26 genes analyzed encode unknown proteins. Genomic locations of the cloned genes and their expressional regulations under the aerobic and anaerobic conditions were also analyzed. These results suggest that this library is useful for the global analysis of the E. coli genomic genes and that this strategy may be applicable to the genomic analysis of other microorganisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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