Wound Fluid-Derived Heparin-Binding EGF-Like Growth Factor (HB-EGF) Is Synergistic with Insulin-Like Growth Factor-I For Balb/MK Keratinocyte Proliferation
Autor: | Elof Eriksson, William G. Taylor, Michael Klagsbrun, Moshe Marikovsky, Peter M. Vogt, Jeffrey S. Rubin, Joachim Sasse |
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Rok vydání: | 1996 |
Předmět: |
Keratinocytes
insulin medicine.medical_specialty Heparin-binding EGF-like growth factor medicine.medical_treatment Molecular Sequence Data Dermatology Biochemistry 3T3 cells Mice Insulin-like growth factor wound repair Epidermal growth factor Internal medicine medicine Animals Humans re-epithelialization Amino Acid Sequence Insulin-Like Growth Factor I Molecular Biology Wound Healing integumentary system Heparin Chemistry Growth factor 3T3 Cells Cell Biology epidermal growth factor medicine.anatomical_structure Endocrinology Immunology Intercellular Signaling Peptides and Proteins Wounds and Injuries Keratinocyte Wound healing Cell Division Heparin-binding EGF-like Growth Factor medicine.drug |
Zdroj: | Journal of Investigative Dermatology. 106(4):616-621 |
ISSN: | 0022-202X |
DOI: | 10.1111/1523-1747.ep12345413 |
Popis: | Epidermal cell proliferation is required for re-epithelialization during wound repair. Re-epithelialization of partial thickness excisional wounds in pigs is complete by 6 days after injury. The presence of insulin-like growth factor-I (IGF-I) and heparin-binding molecules that are mitogenic for keratinocytes was examined in wound fluid obtained daily from these wounds. Two significant heparin-binding growth factor activities for Balb/MK keratinocytes were detected, a major one that was eluted from a heparin affinity column with 1.1 M NaCl and a minor one with 0.5 M NaCl. These activities appeared 1 day after injury, were maximal by 2-3 days later, and disappeared by 6 days after injury. The molecule eluting with 1.1 M NaCl was heparin-binding EGF-like (HB-EGF). The levels of IGF-I in wound fluid were 45-90 ng/ml during the first 3 days following injury, decreased thereafter, and were not detectable 6 days after injury. IGF-I at 100 ng/ml, increased HB-EGF mitogenic activity for Balb/MK keratinocytes by 40-50 fold. We conclude that the synergism between IGF-I and HB-EGF and their relative concentration at the various days after injury may be important variables for regulating re-epithelialization during wound repair. |
Databáze: | OpenAIRE |
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