Wound Fluid-Derived Heparin-Binding EGF-Like Growth Factor (HB-EGF) Is Synergistic with Insulin-Like Growth Factor-I For Balb/MK Keratinocyte Proliferation

Autor: Elof Eriksson, William G. Taylor, Michael Klagsbrun, Moshe Marikovsky, Peter M. Vogt, Jeffrey S. Rubin, Joachim Sasse
Rok vydání: 1996
Předmět:
Zdroj: Journal of Investigative Dermatology. 106(4):616-621
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12345413
Popis: Epidermal cell proliferation is required for re-epithelialization during wound repair. Re-epithelialization of partial thickness excisional wounds in pigs is complete by 6 days after injury. The presence of insulin-like growth factor-I (IGF-I) and heparin-binding molecules that are mitogenic for keratinocytes was examined in wound fluid obtained daily from these wounds. Two significant heparin-binding growth factor activities for Balb/MK keratinocytes were detected, a major one that was eluted from a heparin affinity column with 1.1 M NaCl and a minor one with 0.5 M NaCl. These activities appeared 1 day after injury, were maximal by 2-3 days later, and disappeared by 6 days after injury. The molecule eluting with 1.1 M NaCl was heparin-binding EGF-like (HB-EGF). The levels of IGF-I in wound fluid were 45-90 ng/ml during the first 3 days following injury, decreased thereafter, and were not detectable 6 days after injury. IGF-I at 100 ng/ml, increased HB-EGF mitogenic activity for Balb/MK keratinocytes by 40-50 fold. We conclude that the synergism between IGF-I and HB-EGF and their relative concentration at the various days after injury may be important variables for regulating re-epithelialization during wound repair.
Databáze: OpenAIRE