Oxidative stress on mitochondria and cell membrane of cultured rat hepatocytes and perfused liver exposed to ethanol
| Autor: | Ken Mizukami, Kengo Tomita, Shinzo Kato, N Watanabe, Soichiro Miura, Masahiko Fukuda, Hiromasa Ishii, Hajime Higuchi, Iwao Kurose, Masaaki Takaishi, Yoshitaka Kamegaya, Yoshinori Horie |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Membrane permeability In Vitro Techniques Mitochondrion medicine.disease_cause Permeability Membrane Potentials chemistry.chemical_compound medicine Animals Rats Wistar Ethanol metabolism Cells Cultured Alcohol dehydrogenase Fomepizole Membrane potential Microscopy Confocal Ethanol Hepatology biology Cell Membrane Alcohol Dehydrogenase Thiourea Gastroenterology Free Radical Scavengers Glutathione Mitochondria Rats Perfusion Oxidative Stress Liver Microscopy Fluorescence chemistry Biochemistry Mitochondrial permeability transition pore biology.protein Pyrazoles Oxidative stress |
| Zdroj: | Gastroenterology. 112:1331-1343 |
| ISSN: | 0016-5085 |
| DOI: | 10.1016/s0016-5085(97)70147-1 |
| Popis: | BACKGROUND & AIMS: The precise pathogenic significance of oxidative injury in the evolution of alcohol-induced liver disease is still obscure. The present report was designed to investigate whether ethanol alters the production of active oxidants and biological activities of hepatocytes. METHODS: The following parameters in rat hepatocytes were investigated by using fluorescence probes in vitro and ex vivo: (1) mitochondrial membrane potential and membrane permeability transition, (2) oxygen radicals generation, (3) membrane barrier function, and (4) glutathione level. RESULTS: Ethanol (50 mmol/L) increased oxidative stress in hepatocytes and subsequently induced an increased mitochondrial permeability transition and a decreased membrane potential. These ethanol-induced alterations were attenuated by an inhibitor of alcohol dehydrogenase and an intracellular oxidant scavenger, whereas they were enhanced by diethyl maleic acid, a glutathione depletor. Ethanol plus diethyl maleic acid but not ethanol alone increased the number of hepatocytes with membrane barrier dysfunction. A continuous infusion of ethanol (50 mmol/L) increased oxidative stress and decreased mitochondrial membrane potential in the pericentral area of isolated perfused rat liver. CONCLUSIONS: Active oxidants generated during ethanol metabolism increase mitochondrial permeability transition and modulate mitochondrial energy synthesis in hepatocytes. Reduction of glutathione level enhances mitochondrial dysfunction and impairs membrane barrier function of hepatocytes. (Gastroenterology 1997 Apr;112(4):1331-43) |
| Databáze: | OpenAIRE |
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