Hermetia illucens (Diptera: Stratiomydae) larvae and prepupae: Biomass production, fatty acid profile and expression of key genes involved in lipid metabolism
| Autor: | Cosimo Baviera, Tiziana Cappello, Domenico Savastano, Alessia Giannetto, Fabio de Araújo Pedron, Vincenzo Parrino, Carlos Frederico Ceccon Lanes, Maria Maisano, Giuseppe Lo Paro, Sabrina Oliva, Angela Mauceri, Nunziacarla Spanò, Salvatore Fasulo |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine Hermetia illucens Bioengineering 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences Nutrient 010608 biotechnology Animals Simuliidae Amino Acid Sequence Biomass Food science Lipase Phylogeny Bioconversion Fatty acids Gene characterization Gene expression Lipid metabolism chemistry.chemical_classification biology Fatty Acids fungi Fatty acid General Medicine Lipid Metabolism biology.organism_classification Pyruvate carboxylase Fatty acid synthase 030104 developmental biology chemistry Larva biology.protein Instar Female Sequence Alignment Biotechnology |
| Zdroj: | Journal of Biotechnology. 307:44-54 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2019.10.015 |
| Popis: | The Black Soldier Fly (BSF) Hermetia illucens provides a promising strategy in the waste valorisation process and a sustainable alternative source of valuable nutrients, including lipids for food and feed. In the present study, the differences in growth performances and nutritional values of BSF V instar larvae and prepupae reared on vegetable waste were analyzed and compared focusing on fat content. V instar larvae showed higher capacity to bioconvert the substrate into biomass than prepupae. The nutritional composition and the fatty acid profiles were dependent on the developmental stage. The expression levels of acetyl-CoA carboxylase (acc), fatty acid synthase (fas), lipase (lip) and acyl-CoA dehydrogenase (acd) genes involved in the lipid metabolism pathway and herein characterized for the first time, were evaluated in order to understand the molecular basis underlying the observed differences in fatty acid profiles. Our results suggest that the different fatty acid profiles of BSF V instar larvae and prepupae may be related to the modulation of the lipid metabolism-related genes expression during larval development. Our study highlights substantial differences between H. illucens V instar larvae and prepupae giving important features regarding the opportunity to modulate the preferable fatty acid profile to meet the industrial requirements. |
| Databáze: | OpenAIRE |
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