High-performance liquid chromatographic analysis of human erythrocyte oxysterols as delta 4-3-ketone derivatives
| Autor: | Jon I. Teng, Leland L. Smith |
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| Rok vydání: | 1995 |
| Předmět: |
Hemolytic anemia
Erythrocytes Cholesterol oxidase Anemia Sickle Cell Biochemistry High-performance liquid chromatography Analytical Chemistry Sickle Cell Trait polycyclic compounds medicine Humans Cholestenones Chromatography High Pressure Liquid Sickle cell trait Chromatography integumentary system Chemistry Cholesterol Oxidase organic chemicals Organic Chemistry Erythrocyte Membrane General Medicine Ketones medicine.disease Sterol Thin-layer chromatography Red blood cell Membrane medicine.anatomical_structure |
| Zdroj: | Journal of chromatography. A. 691(1-2) |
| ISSN: | 0021-9673 |
| Popis: | Following oxidation by cholesterol oxidase human erythrocyte oxysterols were analyzed as the corresponding hydroxy-delta 4-3-ketones by HPLC with effluent monitoring at 235 nm. Variable amounts of epimeric cholest-5-ene-3 beta,7-diols, 3 beta-hydroxycholest-5-en-7-one, cholest-5-ene-3 beta,19-diol, cholest-5-ene-3 beta,20-diol, cholest-5-ene-3 beta,25-diol and cholest-5-ene-3 beta,26-diol were detected in erythrocyte membranes of patients with sickle cell anemia or sickle cell trait. Only 3 beta-hydroxycholest-5-en-7-one was detected in erythrocyte membranes of healthy donors. |
| Databáze: | OpenAIRE |
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