Differences in mitogenic responses to angiotensin II, calf serum and phorbol ester in vascular smooth muscle cells from two strains of genetically hypertensive rat
| Autor: | Millar Ja, Grigor Mr, Harris El |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle medicine.medical_treatment Biophysics Peptide hormone Biology Biochemistry Rats Inbred WKY Muscle Smooth Vascular Species Specificity Internal medicine Rats Inbred SHR medicine Animals Molecular Biology Cells Cultured DNA synthesis Cell growth Growth factor Angiotensin II Cell Biology DNA Fetal Blood Growth hormone secretion Stimulation Chemical Culture Media Rats Endocrinology Cell culture Tetradecanoylphorbol Acetate Cattle Cell Division Thymidine |
| Zdroj: | Biochemical and biophysical research communications. 170(3) |
| ISSN: | 0006-291X |
| Popis: | Vascular smooth muscle cells cultured from spontaneously hypertensive (SHR), New Zealand genetically hypertensive (GH) rat strains and their control strains (Wistar-Kyoto (WKY) and normal Wistar (N) rats) were compared for mitogenic responses [( 3H]-thymidine incorporation) to angiotensin II (AII), fetal calf serum (FCS), and the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA). SHR cells showed enhanced basal [3H]-thymidine incorporation and increased responses to all three factors. In contrast, basal and FCS-stimulated [3H]-thymidine incorporation was the same or less in GH than in N cells. However, DNA synthesis was greater in GH cells in response to AII, AII + TPA or FCS + TPA. These results suggest that vascular smooth muscle cells from both hypertensive rat strains display enhanced mitogenesis, but the enhancement occurs via different intracellular signalling pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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