Correlation of increased concentration of ovine endometrial cyclooxygenase 2 with the increase in PGE2 and PGD2 in the late luteal phase

Autor: Tamby Jp, Pierrette Reinaud, Créminon C, Gilles Charpigny
Rok vydání: 1999
Předmět:
Embryology
medicine.medical_specialty
Blotting
Western
Alpha (ethology)
Arachidonic Acids
Luteal Phase
Luteal phase
Endometrium
Dinoprostone
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Estrus
Internal medicine
medicine
Animals
Prostaglandin E2
Cells
Cultured
Chromatography
High Pressure Liquid
030304 developmental biology
Analysis of Variance
0303 health sciences
Sheep
030219 obstetrics & reproductive medicine
biology
Prostaglandin D2
Obstetrics and Gynecology
Cell Biology
Isoenzymes
Thromboxane B2
medicine.anatomical_structure
Reproductive Medicine
chemistry
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Prostaglandins
biology.protein
Female
lipids (amino acids
peptides
and proteins)
Arachidonic acid
Chromatography
Thin Layer
Cyclooxygenase
medicine.drug
Zdroj: Reproduction. 117:315-324
ISSN: 1741-7899
1470-1626
DOI: 10.1530/jrf.0.1170315
Popis: Ovine endometrium showed transient expression of high concentrations of the inducible isoform of cyclooxygenase, cyclooxygenase 2 (COX-2), whereas the constitutive isoform, cyclooxygenase 1 (COX-1), was expressed at much lower concentrations and did not change. In this study, the pattern of prostaglandin synthesis in endometrial luminal cells was investigated in relation to their COX-2 content. Endometrial cells from cyclic or pregnant ewes at days 9, 12, 14 and 16 were isolated and analysed for the presence of COX-1 and COX-2 proteins using western blot analysis. Freshly isolated cells were incubated with 0.5 microCi [3H]arachidonic acid ml-1. Radioactive cyclooxygenase metabolites were analysed by reverse-phase HPLC. Luminal cells produced mainly PGF2 alpha, PGE2, PGD2 and 13,14-dihydro-15-keto PGF2 alpha and to a lesser extent 6-keto PGF1 alpha, thromboxane B2 and 13,14-dihydro-15-keto PGE2. The production of PGE2 and PGD2 was proportional to the cellular concentration of COX-2. PGE2 and PGD2 release was low on day 9 when COX-2 was not expressed, whereas high concentrations of PGE2 and PGD2 were synthesized on days 12-14 when COX-2 was highly expressed, reaching 100 ng microgram-1 cellular protein. In contrast, the basal production of PGF2 alpha did not appear to be related to COX-2 concentration and was greatest on day 16. Moreover, the release of PGF2 alpha was maintained at steady state values between days 9 and 14 by the production of 13,14-dihydro-15-keto PGF2 alpha. Although PGF2 alpha output was lower at day 16 of pregnancy compared with the oestrous cycle, no difference was observed in the pattern of prostaglandin synthesis between pregnant and non-pregnant ewes.
Databáze: OpenAIRE
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