Anillin acts as a bifunctional linker coordinating midbody ring biogenesis during cytokinesis
| Autor: | Silvana Jananji, Yvonne Ruella, Gilles R.X. Hickson, Amel Kechad |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Scaffold protein
Biology Septin Cell junction General Biochemistry Genetics and Molecular Biology Article Cell Line 03 medical and health sciences 0302 clinical medicine Contractile Proteins Animals Drosophila Proteins Cytoskeleton 030304 developmental biology Cytokinesis 0303 health sciences Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) C-terminus Cell Membrane Microfilament Proteins Membrane Drosophila melanogaster Biochemistry Biophysics General Agricultural and Biological Sciences 030217 neurology & neurosurgery Biogenesis |
| Zdroj: | Current biology : CB. 22(3) |
| ISSN: | 1879-0445 |
| Popis: | SummaryAnimal cell cytokinesis proceeds via constriction of an actomyosin-based contractile ring (CR) [1, 2]. Upon reaching a diameter of ∼1 μm [3], a midbody ring (MR) forms to stabilize the intercellular bridge until abscission [4–6]. How MR formation is coupled to CR closure and how plasma membrane anchoring is maintained at this key transition is unknown. Time-lapse microscopy of Drosophila S2 cells depleted of the scaffold protein Anillin [7–9] revealed that Anillin is required for complete closure of the CR and formation of the MR. Truncation analysis revealed that Anillin N termini connected with the actomyosin CR and supported formation of stable MR-like structures, but these could not maintain anchoring of the plasma membrane. Conversely, Anillin C termini failed to connect with the CR or MR but recruited the septin Peanut to ectopic structures at the equatorial cortex. Peanut depletion mimicked truncation of the Anillin C terminus, resulting in MR-like structures that failed to anchor the membrane. These data demonstrate that Anillin coordinates the transition from CR to MR and that it does so by linking two distinct cortical cytoskeletal elements. One apparently acts as the core structural template for MR assembly, while the other ensures stable anchoring of the plasma membrane beyond the CR stage. |
| Databáze: | OpenAIRE |
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