Raman imaging of rat nonalcoholic fatty liver tissues reveals distinct biomolecular states

Autor: Khalifa Mohammad Helal, Harsono Cahyadi, J. Nicholas Taylor, Akira Okajima, Koji Tabata, Yasuaki Kumamoto, Kentaro Mochizuki, Yoshito Itoh, Tetsuro Takamatsu, Hideo Tanaka, Katsumasa Fujita, Tamiki Komatsuzaki, Yoshinori Harada
Rok vydání: 2023
Předmět:
Zdroj: FEBS Letters.
ISSN: 1873-3468
0014-5793
Popis: An essential challenge in diagnosing states of nonalcoholic fatty liver disease (NAFLD) is the early prediction of progression from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH) before the disease progresses. Histological diagnoses of NAFLD rely on the appearance of anomalous tissue morphologies, and it is difficult to segment the biomolecular environment of the tissue through a conventional histopathological approach. Here, we show that hyperspectral Raman imaging provides diagnostic information on NAFLD in rats, as spectral changes among disease states can be detected before histological characteristics emerge. Our results demonstrate that Raman imaging of NAFLD can be a useful tool for histopathologists, offering biomolecular distinctions among tissue states that cannot be observed through standard histopathological means.
Databáze: OpenAIRE
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