Nitric Oxide Release from Antimicrobial Peptide Hydrogels for Wound Healing
| Autor: | Cristina C. Barrias, Nuno Vale, Luís Gales, Joana Durão, Salome Gomes, Paula Gomes |
|---|---|
| Přispěvatelé: | Instituto de Investigação e Inovação em Saúde |
| Rok vydání: | 2018 |
| Předmět: |
collagen
Fibroblasts / drug effects Lysine lcsh:QR1-502 Peptide 02 engineering and technology Antimicrobial activity 01 natural sciences Biochemistry lcsh:Microbiology NO release chemistry.chemical_compound Griess test Antimicrobial Cationic Peptides / chemical synthesis chemistry.chemical_classification Chemistry Escherichia coli / drug effects Fibroblasts / metabolismo Hydrogels Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Antimicrobial 3. Good health Self-healing hydrogels Hydrogels / chemistry Collagen Antimicrobial Cationic Peptides / pharmacology 0210 nano-technology Fmoc-Pexiganan Nitric Oxide 010402 general chemistry Article Cell Line Nitric oxide Escherichia coli Humans Antimicrobial Cationic Peptides / chemistry Molecular Biology Wound Healing antimicrobial activity Collagen / metabolismo Fibroblasts / cytology Nitric Oxide / metabolismo Fibroblasts 0104 chemical sciences Wound Healing / drug effects Kinetics Ninhydrin Wound healing Antimicrobial Cationic Peptides |
| Zdroj: | Biomolecules Volume 9 Issue 1 Biomolecules, Vol 9, Iss 1, p 4 (2018) |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom9010004 |
| Popis: | Nitric oxide (NO) is an endogenously produced molecule that has been implicated in several wound healing mechanisms. Its topical delivery may improve healing in acute or chronic wounds. In this study an antimicrobial peptide was synthesized which self-assembled upon a pH shift, forming a hydrogel. The peptide was chemically functionalized to incorporate a NO-donor moiety on lysine residues. The extent of the reaction was measured by ninhydrin assay and the NO release rate was quantified via the Griess reaction method. The resulting compound was evaluated for its antimicrobial activity against Escherichia coli, and its effect on collagen production by fibroblasts was assessed. Time-kill curves point to an initial increase in bactericidal activity of the functionalized peptide, and collagen production by human dermal fibroblasts when incubated with the NO-functionalized peptide showed a dose-dependent increase in the presence of the NO donor within a range of 0–20 µM. This work was financed by FEDER (Fundo Europeu de Desenvolvimento Regional) funds via COMPETE 2020 (Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020), and by Portuguese funds through FCT (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia e Ensino Superior) in the framework of the projects “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274) and PTDC/QUI-QFI/29914/2017, as well through the grant SFRH/BD/84914/2012. Thanks to FCT also for supporting Research Unit LAQV-REQUIMTE through the project UID/QUI/5006/2013. |
| Databáze: | OpenAIRE |
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