Low molecular weight RNA associated with 28 s nucleolar RNA
| Autor: | Harris Busch, Archie W. Prestayko, Maurizio Tonato |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Male
Nucleotides 5.8S ribosomal RNA RNA Nucleosides Neoplasms Experimental Biology Electrophoresis Disc Non-coding RNA Ribosome Rats Post-transcriptional modification Molecular Weight Crystallography Biochemistry Structural Biology Preribosomal RNA Centrifugation Density Gradient RNA polymerase I Animals RNA Neoplasm Ribosomes Molecular Biology Cell Nucleolus Small nuclear RNA |
| Zdroj: | Journal of Molecular Biology. 47:505-515 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(70)90318-9 |
| Popis: | Extraction of nucleolar RNA at 65 °C results in the liberation from nucleolar 28 s RNA of a hitherto undescribed low molecular weight RNA referred to as 8 s (28 s) RNA, as well as a previously described U3 RNA and a 7 s RNA (Pene, Knight & Darnell, 1968; Weinberg & Penman, 1968). The U3 and 8 s (28 s) RNA's are not degradation or cleavage products since their rates of labeling with [ 32 P]orthophosphate are lower than those of nucleolar RNA's of higher molecular weight. The U3 and 8 s (28 s) nucleolar RNA's differ in composition and mobility from the ribosomal and nucleolar 7 s RNA thermal products. Several molecular species appear to be present in the U3 and 8 s (28 s) RNA's, since the major 3′-terminal nucleosides of U3 RNA are adenosine and uridine and the major 3′-terminal nucleosides of 8 s (28 s) RNA are cytidine and uridine. Interestingly, neither nucleolar 45 s RNA nor 35 s RNA contains the U3 or 8 s RNA components which are present in the nucleolar 28 s RNA. |
| Databáze: | OpenAIRE |
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