The enhancement of neutron irradiation of HeLa-S cervix carcinoma cells by cell–nucleus-addressed deca-p-boronophenylalanine
| Autor: | Waldemar Waldeck, Andreas Eisenmenger, Gerd Wolber, Klaus Braun, Isabell Braun, Ralf Rastert, Joürgen Debus, Stefan Heckl, Rüdiger Pipkorn, Jürgen Jenne, Heike Corban-Wilhelm, Volker Ehemann |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Boron Compounds
inorganic chemicals Radiation-Sensitizing Agents Cell Survival Phenylalanine medicine.medical_treatment Molecular Sequence Data Active Transport Cell Nucleus Uterine Cervical Neoplasms Linear energy transfer chemistry.chemical_element Boron Neutron Capture Therapy Mass Spectrometry HeLa Drug Discovery medicine Humans Amino Acid Sequence Boron Cell Nucleus Pharmacology Microscopy Confocal biology Carcinoma Organic Chemistry Radiochemistry technology industry and agriculture General Medicine Flow Cytometry biology.organism_classification Neutron temperature Radiation therapy Neutron capture Cell killing chemistry Cell culture Female HeLa Cells |
| Zdroj: | European Journal of Medicinal Chemistry. 38:587-595 |
| ISSN: | 0223-5234 |
| Popis: | Boron neutron capture therapy (BNCT) is an experimental treatment modality which depends on a sufficient cellular uptake of Boron ((10)B) followed by an exposure to a thermal neutron beam from a nuclear reactor. High energetic particles (4He and 7Li) are created during the neutron capture reaction and produce DNA damages, which lead to cell killing. Regarding BNCT, the short radiation range of He- and Li-particles is decisive for the distribution of (10)B. Until now, BNCT has been lacking for therapeutically effective concentrations of (10)B. Twenty-four hours after the combined use of our 'Bioshuttle'-p-borono-phenylalanine(10)-constructs ('Bioshuttle'-p-BPA(10)) and neutron-irradiation, an obvious reduction of the radiation-resistant HeLa-S cells could be observed. No cells were alive 72 h after the incubation with 'Bioshuttle'-p-BPA(10) followed by neutron irradiation. A post-mitotic cell death could be assumed based on flow cytometrical data. |
| Databáze: | OpenAIRE |
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