Cytotoxicity of Carbon Nanotubes on J774 Macrophages Is a Purification-Dependent Effect

Autor:	Erasmo Orrantia-Borunda, Antonia Luna-Velasco, Carmen González Horta, A. Duarte-Moller, Silvia Lorena Montes-Fonseca, Blanca Sánchez-Ramírez, M. Román-Aguirre
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	Materials science Article Subject Scanning electron microscope Sonication Carbon nanotube law.invention symbols.namesake law Toxicity lcsh:Technology (General) symbols Organic chemistry Particle lcsh:T1-995 General Materials Science Titration Cytotoxicity Raman spectroscopy Nuclear chemistry
Zdroj:	Journal of Nanomaterials, Vol 2012 (2012)
ISSN:	1687-4129 1687-4110
Popis:	The cytotoxicity of the carbon nanotubes (CNTs) is an important factor for the manufacture of nanovaccines. The aim of this work was to evaluate the relationship of the purification method of CNTs in cellular toxicity using macrophages (MOs) from the J774 cell line. Viability test was performed with MTT assays at 24 h of exposure at concentrations of 0.06, 0.6, and 6 mg/L of unpurified (UP-CNTs) or purified (P-CNTs) CNTs by two different methods: (1) reflux with 3M HNO3and (2) sonication in H2SO4/HNO3. Characterization and COOH content of CNTs was performed using scanning electron microscopy, raman spectroscopy, and titration with NaHCO3. P-CNTs1had lengths >100 μm and 2.76% COOH content, while P-CNTs2had lengths >1 μm and 7% COOH content. This last particle showed a lower toxic effect. The results suggest that the lenght and COOH content are important factors in the toxicity of the CNTs.
Databáze:	OpenAIRE
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