Displacement effect during HPLC preparative purification of human insulin
| Autor: | V. A. Lasman, V. D. Gusarova, V. Nekipelova, D. A. Gusarov, D. I. Bairamashvili |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
INSULIN HUMAN
Sorbent Chromatography Chemistry Insulin medicine.medical_treatment Clinical Biochemistry Cell Biology General Medicine Hydrogen-Ion Concentration Biochemistry High-performance liquid chromatography Process scale Analytical Chemistry Pharmaceutical technology medicine Human insulin Humans Hypoglycemic Agents Indicators and Reagents Drug Contamination Displacement (fluid) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B. 877:1216-1220 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2009.02.062 |
| Popis: | HPLC plays a key role in the preparative purification of human insulin. A21-desamidoinsulin is one of the impurities that possesses the chromatographic behavior similar to that of insulin and hence separation from this by-product is rather difficult at the process scale. During the optimization of insulin reversed-phase HPLC purification, when a column was sufficiently overloaded, the effect of displacement of A21-desamidoinsulin molecules from active groups of sorbent by insulin ones was observed. It was suggested that monocarboxylic acid and organic modifier in mobile phase are responsible for the esterification during which the formed ester promotes the displacement effect. This effect was studied in order to optimize the purification of human insulin at the process scale. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect