In Vitro Experimental Model of Trained Innate Immunity in Human Primary Monocytes
| Autor: | Siroon Bekkering, Bastiaan A. Blok, Leo A. B. Joosten, Niels P. Riksen, Reinout van Crevel, Mihai G. Netea |
|---|---|
| Přispěvatelé: | Experimental Vascular Medicine |
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine beta-Glucans medicine.medical_treatment Clinical Biochemistry lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] Lipoproteins LDL/immunology Cell morphology Monocytes Cytokines/biosynthesis 0302 clinical medicine Immunology and Allergy Cells Cultured BCG Vaccine/immunology chemistry.chemical_classification Microscopy Confocal Vascular damage Radboud Institute for Molecular Life Sciences [Radboudumc 16] Warburg effect 3. Good health Lipoproteins LDL medicine.anatomical_structure Cytokine 030220 oncology & carcinogenesis BCG Vaccine Cytokines Glycolysis Microbiology (medical) Immunology education Biology 03 medical and health sciences Reactive Oxygen Species/metabolism Immunity medicine Humans Lipopolysaccharides/immunology Author Correction Reactive oxygen species Innate immune system Monocyte Monocytes/immunology Immunity Innate In vitro beta-Glucans/immunology lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4] 030104 developmental biology chemistry Reactive Oxygen Species |
| Zdroj: | Clinical and Vaccine Immunology, 23, 926-933 Clinical and vaccine immunology, 23(12), 926-933. American Society for Microbiology Bekkering, S, Blok, B A, Joosten, L A B, Riksen, N P, van Crevel, R & Netea, M G 2016, ' In Vitro experimental model of trained innate immunity in human primary monocytes ', Clinical and Vaccine Immunology (Online), vol. 23, no. 12, pp. 926-933 . https://doi.org/10.1128/CVI.00349-16 Clinical and Vaccine Immunology, 23, 12, pp. 926-933 Clinical and Vaccine Immunology |
| ISSN: | 1556-6811 |
| Popis: | Innate immune memory, or trained immunity, has recently been described to be an important property of cells of the innate immune system. Due to the increased interest in this important new field of immunological investigation, we sought to determine the optimal conditions for an in vitro experimental protocol of monocyte training using three of the most commonly used training stimuli from the literature: β-glucan, the bacillus Calmette-Guérin (BCG) vaccine, and oxidized low-density lipoprotein (oxLDL). We investigated and optimized a protocol of monocyte trained immunity induced by an initial training period with β-glucan, BCG, or oxLDL, followed by washing and resting of the cells and, thereafter, restimulation with secondary bacterial stimuli. The training and resting time intervals were varied to identify the optimal setting for the long-term induction of trained immunity. Trained immunity was assessed in terms of the secondary cytokine response, the production of reactive oxygen species, cell morphology, and induction of glycolysis. Monocytes primed with β-glucan, BCG, and oxLDL showed increased pro- and anti-inflammatory cytokine responses upon restimulation with nonrelated stimuli. Also, all three stimuli induced a switch to glycolysis (the Warburg effect). These effects were most pronounced when the training interval was 24 h and the resting time interval was 6 days. Training with BCG and oxLDL also led to the increased production of reactive oxygen species, whereas training with β-glucan led to the decreased production of reactive oxygen species. We describe the optimal conditions for an in vitro experimental model with human primary monocytes for study of the induction of trained innate immunity by microbial and metabolic stimuli. |
| Databáze: | OpenAIRE |
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