Evaluation of Seven Commercial ELISA Kits Compared with the C1q Solid-Phase Binding RIA for Detection of Circulating Immune Complexes
| Autor: | Ernestina Van Hoeyveld, Xavier Bossuyt |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Systemic disease
Antigen-Antibody Complex business.industry Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Enzyme-Linked Immunosorbent Assay Reference Standards medicine.disease Sensitivity and Specificity Immune complex Raji cell assay Immune system Immunopathology Clinical information Immunology medicine Humans Reagent Kits Diagnostic business |
| Zdroj: | Clinical Chemistry. 46:283-285 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Circulating immune complexes (CICs) are detectable in a variety of systemic disorders such as rheumatological and autoimmune diseases (1)(2)(3)(4); allergic diseases (5); viral, bacterial, and parasitic infections (1)(6)(7)(8); and malignancies (2)(9). Although detection of CICs is neither essential nor specific for any disease, it may provide useful clinical information regarding immunopathology, prognosis, and follow-up of rheumatic and autoimmune disorders. A variety of tests that can detect CICs have been described (10)(11)(12)(13)(14)(15)(16)(17)(18). The assays that are used routinely in clinical laboratories are based on (a) precipitation of CICs by polyethylene glycol, (b) interaction of CICs with complement (C1q assay), (c) detection of CICs bound to C3 (Raji cell assay, conglutinin assay), and (d) binding of CICs to Fc-recognizing molecules (mRF assay). In a comparative WHO study (10), only a few tests were described as sufficiently sensitive and reproducible. This study revealed that the C1q solid-phase RIA (C1q-SP RIA) discriminated well between sera from healthy persons and those from patients. Several studies have indicated that this assay correlated better with disease activity than do other immune complex tests (8). The clinical activity of systemic lupus erythematosus correlated well with the concentration of CICs in the solid-phase RIA (19)(20). Subsequently, this assay has been used frequently in clinical investigations. The working group therefore concluded that the C1q-SP test was one of the most reliable tests. For these reasons, we used this test as the reference method. Several ELISA kits have become commercially available … |
| Databáze: | OpenAIRE |
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