SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum
| Autor: | Lisa K. Rainville, Andrew H. Paterson, Dong Zhang, Brent Kronmiller, Susan A. Auckland, Changsoo Kim, Erik J. Sacks, Katrin Jakob, Martin Deuter |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Genetic Markers
biology DNA Plant Genetic Linkage food and beverages Chromosome Mapping Miscanthus sinensis General Medicine Miscanthus biology.organism_classification Sorghum Genome Miscanthus sacchariflorus Saccharum Gene mapping Genetic marker Botany Genetics Microsatellite Agronomy and Crop Science Sequence Alignment Biotechnology Microsatellite Repeats |
| Zdroj: | TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 124(7) |
| ISSN: | 1432-2242 |
| Popis: | We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus × giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication. While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink