Isolation and culture of human hepatocytes from resected liver tissue as a bioreactor for a hybrid artificial liver
| Autor: | Yasuaki Nakajima, Matsushita M, Hirokazu Matsue, Junichi Uchino, Manabu Takahashi |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Cell Survival Biomedical Engineering Medicine (miscellaneous) Bioengineering Biology Biomaterials chemistry.chemical_compound Dispase medicine Bioreactor Humans Urea Cells Cultured Warm Ischemia Time Proteolytic enzymes Gluconeogenesis General Medicine DNA Middle Aged Molecular biology chemistry Liver Reperfusion Injury Collagenase Female Artificial Organs Perfusion medicine.drug |
| Zdroj: | Artificial organs. 17(7) |
| ISSN: | 0160-564X |
| Popis: | To use cultured human hepatocytes as a hybrid artificial liver, effective methods for isolating and culturing the hepatocytes from resected surgical specimens were investigated. Two different procedures for isolating hepatocytes, perfusion and agitation with a collagenase solution (Method 1) and perfusion with a mixed solution of collagenase and dispase (Method 2), were examined. The yield of isolated hepatocytes obtained by Method 2 (13.31 times 106 cellslg of liver) was significantly higher than that by Method 1 (0.94 times 106). The warm ischemia time (0–90 min) of the liver fragments obtained did not disturb the viability and yield of the isolated hepatocytes. The gluconeogenesis and urea synthesis of the cultured human hepatocytes were well preserved for 10 days. These results show that for prolonged human hepatocyte culture (10 days), isolation from resected human liver tissues by a combination of the proteolytic enzymes collagenase and dispase was effective and warm ischemia was tolerated for up to 90 min, which indicates the possibility of using cultured human hepatocytes as a hybrid artificial liver. |
| Databáze: | OpenAIRE |
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