Synthesis and characterization of surface-grafted polyacrylamide brushes and their inhibition of microbial adhesion
| Autor: | Henk J. Busscher, Jeroen Luijten, Arend Jan Schouten, Henny C. van der Mei, Irina Cringus-Fundeanu |
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| Přispěvatelé: | Polymers at Surfaces and Interfaces |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Surface Properties
Staphylococcus Radical polymerization Polyacrylamide Acrylic Resins GLASS Polymer brush MONOLAYER Bacterial Adhesion LAYERS chemistry.chemical_compound BIOMATERIAL Polymer chemistry Candida albicans Electrochemistry General Materials Science Fourier transform infrared spectroscopy Particle Size SILICONE-RUBBER Spectroscopy chemistry.chemical_classification Molecular Structure Atom-transfer radical-polymerization Chemistry technology industry and agriculture YEASTS Surfaces and Interfaces Polymer Adhesion Condensed Matter Physics ATTACHMENT Polymerization DENSITY BACTERIA RADICAL POLYMERIZATION |
| Zdroj: | Langmuir, 23(9), 5120-5126. AMER CHEMICAL SOC |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la063531v |
| Popis: | A method is presented to prevent microbial adhesion to solid surfaces exploiting the unique properties of polymer brushes. Polyacrylamide (PAAm) brushes were grown from silicon wafers by atom transfer radical polymerization (ATRP) using a three-step reaction procedure consisting of immobilization of a coupling agent gamma-aminopropyltriethoxysilane, anchoring of an ATRP initiator 4-(chloromethyl)benzoyl chloride, and controlled radical polymerization of acrylamide. The surfaces were characterized by X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, ellipsometry, and contact-angle measurements. The calculated grafting density pointed to the presence of a dense and homogeneous polymer brush. Initial deposition rates, adhesion after 4 h, and detachment of two bacterial strains (Staphylococcus aureus ATCC 12600 and Streptococcus salivarius GB 24/9) and one yeast strain (Candida albicans GB 1/2) to both PAAm-coated and untreated silicon surfaces were investigated in a parallel plate flow chamber. A high reduction (70-92%) in microbial adhesion to the surface-grafted PAAm brush was observed, as compared with untreated silicon surfaces. Application of the proposed grafting method to silicone rubbers may offer great potential to prevent biomaterials-centered infection of implants. |
| Databáze: | OpenAIRE |
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