PIPPin, a Putative RNA-Binding Protein Specifically Expressed in the Rat Brain
| Autor: | Maria Scaturro, Daniele Castiglia, Tommaso Nastasi, Italia Di Liegro, A. Cestelli |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Polyadenylation Molecular Sequence Data Restriction Mapping Biophysics Gene Expression Nerve Tissue Proteins RNA-binding protein Biochemistry Complementary DNA Consensus Sequence Animals Humans Amino Acid Sequence RNA Messenger Molecular Biology Gene Conserved Sequence Gene Library Southern blot Cloning Base Sequence Sequence Homology Amino Acid biology Brain RNA-Binding Proteins RNA Cell Biology biology.organism_classification Molecular biology Rats Drosophila melanogaster |
| Zdroj: | Biochemical and Biophysical Research Communications. 218:390-394 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.0068 |
| Popis: | In looking for genes encoding RNA-binding factors, we prepared an expression library in lambda gt11, by cloning cDNAs corresponding to the polyadenylated fraction of RNA from rat brain at the embryonal day 18. The library was then screened by binding to a radioactive RNA, transcribed in vitro from a cDNA encoding the rat histone variant H3.3. Here we report some findings concerning a cDNA for a protein which contains two putative double stranded RNA-binding domains (dsBD). The corresponding message is specifically expressed in the brain. Moreover, Southern blot analysis showed that the gene is highly conserved from Drosophila melanogaster to man. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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