Dietary effects of succinic acid on the growth, digestive enzymes, immune response and resistance to ammonia stress of Litopenaeus vannamei
| Autor: | Yafei Duan, Jiasong Zhang, Jun Wang, Yun Wang, Yongxu Sun |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Feed additive Litopenaeus Succinic Acid Aquatic Science Arthropod Proteins 03 medical and health sciences chemistry.chemical_compound Pepsin Penaeidae Ammonia Stress Physiological Environmental Chemistry Animals Amylase Food science chemistry.chemical_classification biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Animal Feed Immunity Innate Shrimp Diet Gastrointestinal Tract 030104 developmental biology Enzyme chemistry Succinic acid Dietary Supplements 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Hepatopancreas |
| Zdroj: | Fishshellfish immunology. 78 |
| ISSN: | 1095-9947 |
| Popis: | Organic acids acts as an growth promoter and antimicrobial agent in aquaculture. The present study investigated the effects of a natural organic acid - succinic acid (SA) on the growth, digestive enzymes, immune response and resistance to ammonia stress of Litopenaeus vannamei. The shrimps were firstly fed with diets containing different levels of SA: 0% (Control), 0.25% (SA1), 0.50% (SA2), and 1.0% (SA3) (w/w) for 56 days, followed by an acute ammonia stress for 48 h. The results indicated that dietary of SA improved the growth of shrimp, and increased the survival rate of shrimp after ammonia stress for 48 h. The amylase, lipase and pepsin activity increased in hepatopancreas in three SA group, while trypsin activity was only increased in the SA1 and SA2 groups. At 56 d, T-NOS activity, proPO and HSP70 gene expression level increased in the three SA group, PO activity increased in the SA1 and SA2 groups, T-AOC content and Toll gene expression level increased in the SA2 and SA3 groups, Trx and SOD gene expression level increased in the SA2 group, while Imd, GS and GDH gene expression level was no changes. After exposure to ammonia stress for 48 h, immune biochemical parameters (T-AOC and PO) and genes (proPO, HSP70, Trx and GDH) expression level increased in the three SA group, T-NOS activity, Toll, Imd and GS gene expression level increased in the SA2 and SA3 groups, while SOD gene expression level increased in the SA1 and SA2 groups. These results indicated that SA improved growth, enhanced digestive and immune capacities of L. vannamei against ammonia stress, and may be a potential feed additive for shrimp. The optimal dietary supplementation dosage is 0.50% (w/w) in diet. |
| Databáze: | OpenAIRE |
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