Pseudomonas floridensis sp. nov., a bacterial pathogen isolated from tomato
Autor: | Erica M. Goss, James F. Preston, Matthews L. Paret, Sujan Timilsina, Eric A. Newberry, Gerald V. Minsavage, Jeffrey B. Jones, Gary E. Vallad |
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Rok vydání: | 2018 |
Předmět: |
DNA
Bacterial 0301 basic medicine Sequence analysis 030106 microbiology Microbiology 03 medical and health sciences Solanum lycopersicum Pseudomonas RNA Ribosomal 16S Tobacco Pseudomonas syringae Phylogeny Ecology Evolution Behavior and Systematics Plant Diseases Solanum tuberosum Base Composition biology Strain (chemistry) Pseudomonas viridiflava Fatty Acids Nucleic Acid Hybridization Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Housekeeping gene Genes Bacterial Multilocus sequence typing Multilocus Sequence Typing |
Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 68:64-70 |
ISSN: | 1466-5034 1466-5026 |
Popis: | An unusual fluorescent pseudomonad was isolated from tomato exhibiting leaf spot symptoms similar to bacterial speck. Strains were fluorescent, oxidase- and arginine-dihydrolase-negative, elicited a hypersensitive reaction on tobacco and produced a soft rot on potato slices. However, the strains produced an unusual yellow, mucoid growth on media containing 5 % sucrose that is not typical of levan. Based on multilocus sequence analysis using 16S rRNA, gap1, gltA, gyrB and rpoD, these strains formed a distinct phylogenetic group in the genus Pseudomonas and were most closely related to Pseudomonas viridiflava within the Pseudomonas syringae complex. Whole-genome comparisons, using average nucleotide identity based on blast, of representative strain GEV388T and publicly available genomes representing the genus Pseudomonas revealed phylogroup 7 P. viridiflava strain UASW0038 and P. viridiflava type strain ICMP 2848T as the closest relatives with 86.59 and 86.56 % nucleotide identity, respectively. In silico DNA–DNA hybridization using the genome-to-genome distance calculation method estimated 31.1 % DNA relatedness between GEV388T and P. viridiflava ATCC 13223T, strongly suggesting the strains are representatives of different species. These results together with Biolog GEN III tests, fatty acid methyl ester profiles and phylogenetic analysis using 16S rRNA and multiple housekeeping gene sequences demonstrated that this group represents a novel species member of the genus Pseudomonas . The name Pseudomonas floridensis sp. nov. is proposed with GEV388T (=LMG 30013T=ATCC TSD-90T) as the type strain. |
Databáze: | OpenAIRE |
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