Alternative Variants of Human HYDIN Are Novel Cancer-Associated Antigens Recognized by Adaptive Immunity
| Autor: | Barbara Schmid-Horch, Steffen Walter, Stefan Stevanovic, P. V. Belousov, Ludger Grosse-Hovest, Alexey Y. Sazykin, Dmitry V. Kuprash, Dmitry Penkov, Karoline Laske, Ekaterina P. Koroleva, Sergei A. Nedospasov, Svetlana V. Khlgatian, Hans-Georg Rammensee, Yuriy V. Shebzukhov, David Eisel, Verona Vass, Cécile Gouttefangeas |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Immunology Adaptive Immunity CD8-Positive T-Lymphocytes medicine.disease_cause Mice Chromosome 16 Antigen Antigens Neoplasm Neoplasms Immunoscreening HLA-A2 Antigen medicine Animals Humans RNA Messenger Genetics Mutation biology Microfilament Proteins Cancer medicine.disease Acquired immune system Alternative Splicing Chromosomes Human Pair 1 biology.protein Antibody Chromosomes Human Pair 16 CD8 |
| Zdroj: | Cancer Immunology Research. 1:190-200 |
| ISSN: | 2326-6074 2326-6066 |
| Popis: | A mutation in the hydin gene has been recently described as one possible mechanism leading to lethal congenital hydrocephalus in mice, and a similar defect is proposed to be involved in an autosomal recessive form of hydrocephalus in human. Here, we report for the first time on the cancer association and immunogenicity of two HYDIN variants in humans. One is a previously described sequence derived from the chromosome 1 gene copy, that is, KIAA1864. The second is encoded by a novel alternative transcript originating from the chromosome 16, which we identified by immunoscreening of a testis-derived cDNA expression library with sera of patients with colorectal cancer, and called MO-TES391. Both variants are targeted by immunoglobulin G antibodies in a significant subset of cancer patients but only rarely in healthy donors. Moreover, we identify HLA-A*0201–restricted sequences derived from MO-TES391 and KIAA1864, which are specifically recognized by human cytotoxic CD8+ T cells. Taken together, these results suggest frequent and coordinated adaptive immune responses against HYDIN variants in patients with cancer and propose HYDIN as a novel cancer-associated antigen. Cancer Immunol Res; 1(3); 190–200. ©2013 AACR. |
| Databáze: | OpenAIRE |
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