Effect of the Association and Evaluation of the Induction to Adaptation of the (+)-α-pinene with Commercial Antimicrobials against Strains of Escherichia coli
| Autor: | Zilka Nanes Lima, Francisco Fábio Marques da Silva, Sávio Ferreira, Felipe Lemos Esteves do Amaral, Paula Benvindo Ferreira, Thamara Rodrigues de Melo, Raquel Carlos de Brito, Ticiane Farias |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
Cefepime Antibiotics Ceftazidime Microbial Sensitivity Tests Biology medicine.disease_cause 01 natural sciences Microbiology Cefoxitin 03 medical and health sciences Minimum inhibitory concentration Drug Discovery Escherichia coli medicine Amikacin Bicyclic Monoterpenes 030304 developmental biology 0303 health sciences Amoxicillin General Medicine Antimicrobial Anti-Bacterial Agents 0104 chemical sciences 010404 medicinal & biomolecular chemistry medicine.drug |
| Zdroj: | Current Topics in Medicinal Chemistry. 20:2300-2307 |
| ISSN: | 1568-0266 |
| DOI: | 10.2174/1568026620666200820150425 |
| Popis: | Background: The increasing and inappropriate use of antibiotics has increased the number of multidrug-resistant microorganisms to these drugs, causing the emergence of infections that are difficult to control and manage by health professionals. As an alternative to combat these pathogens, some monoterpenes have harmful effects on the bacterial cell membrane, showing themselves as an alternative in combating microorganisms. Therefore, the positive enantiomer α -pinene becomes an alternative to fight bacteria, since it was able to inhibit the growth of the species Escherichia coli ATCC 25922, demonstrating the possibility of its use as an isolated antimicrobial or associated with other drugs. Aims: The aim of this study is to evaluate the sensitivity profile of E. coli ATCC 25922 strain against clinical antimicrobials associated with (+) -α-pinene and how it behaves after successive exposures to subinhibitory concentrations of the phytochemicals. Methods: : The minimum inhibitory concentration (MIC) was determined using the microdilution method. The study of the modulating effect of (+) -α-pinene on the activity of antibiotics for clinical use in strains of E. coli and the analysis of the strain's adaptation to the monoterpene were tested using the adapted disk-diffusion method. Results: The results demonstrate that the association of monoterpene with the antimicrobials ceftazidime, amoxicillin, cefepime, cefoxitin and amikacin is positive since it leads to the potentiation of the antibiotic effect of these compounds. It was observed that the monoterpene was able to induce crossresistance only for antimicrobials: cefuroxime, ceftazidime, cefepime and chloramphenicol. Conclusion: It is necessary to obtain more concrete data for the safe use of these combinations, paying attention to the existence of some type of existing toxicity reaction related to the herbal medicine and to understand the resistance mechanisms acquired by the microorganism. |
| Databáze: | OpenAIRE |
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