Viral spliced RNA are produced, encapsidated and reverse transcribed during in Vivo woodchuck hepatitis virus infection
| Autor: | Olivier Hantz, Christian Trepo, Isabelle Chemin, I. Baginski, I. Fourel |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Transcription
Genetic RNA Splicing viruses Molecular Sequence Data RNA-dependent RNA polymerase Hepadnaviridae Polymerase Chain Reaction Transcription (biology) Virology Animals Amino Acid Sequence Cloning Molecular Gene Base Sequence biology Woodchuck hepatitis virus Intron RNA RNA-Directed DNA Polymerase biochemical phenomena metabolism and nutrition Blotting Northern biology.organism_classification Molecular biology digestive system diseases Reverse transcriptase Blotting Southern Hepatitis Viral Animal Marmota DNA Viral RNA splicing RNA Viral |
| Zdroj: | Virology. 190:193-200 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(92)91205-9 |
| Popis: | By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|