Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation
| Autor: | Lingpeng Zhan, Wengang Chai, Jinyu Zhou, Yan Li, Huanyu Gao, Min Zhao, Wenchun Xie, Chuncui Huang, Jingyu Yan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Stereochemistry
Electrospray ionization Oligosaccharides 02 engineering and technology Mass spectrometry Tandem mass spectrometry 01 natural sciences Biochemistry Dissociation (chemistry) Analytical Chemistry ABO Blood-Group System Lewis Blood Group Antigens Fragmentation (mass spectrometry) Tandem Mass Spectrometry Ionization Environmental Chemistry Molecule Glucans Spectroscopy chemistry.chemical_classification Chemistry 010401 analytical chemistry Oligosaccharide 021001 nanoscience & nanotechnology 0104 chemical sciences Blood Grouping and Crossmatching Carbohydrate Sequence Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization 0210 nano-technology Sequence Analysis |
| Zdroj: | Analytica chimica acta. 1071 |
| ISSN: | 1873-4324 |
| Popis: | Mass spectrometry (MS) has become the primary method for high-sensitivity structural determination of oligosaccharides. Fragmentation in the negative-ion MS can provide a wealth of structural information and these can be used for sequence determination. However, although negative-ion MS of neutral oligosaccharide using the deprotonated molecule [M-H]- as the precursor has been very successful for electrospray ionization (ESI), it has only limited success for matrix-assisted laser desorption/ionization (MALDI). In the present study, the features of negative-ion MALDI primary spectra were investigated in detail and the product-ion spectra using [M-H]- and [M+Cl]- as the precursors were carefully compared. The formation of [M-H]- was the main difficulty for MALDI while [M+Cl]- was proved to be useful as alternative precursor anion for MALDI-MS/MS to produce similar fragmentation for sequencing of neutral oligosaccharides. N-(1-naphthyl)ethylenediamine dihydrochloride was then used as both the matrix and the Cl- dopant to evaluate the extent of structural information that can be obtained by negative-ion fragmentation from [M+Cl]- using laser-induced dissociation (LID)-MS/MS for linkage assignment of gluco-oligosaccharides and for typing of blood-group ABO(H) and Lewis antigens on either type 1 or type 2 backbone-chains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect