Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo
| Autor: | Natalia Girola, Camyla Fernandez de Farias, Luiz R. Travassos, Mariana H. Massaoka, Felipe V. Pereira, Elaine G. Rodrigues, Aline Nogueira Rabaca, Ricardo A. Azevedo, Luciana Pereira Silva, João Henrique G. Lago, Regildo Márico Gonçalves da Silva, Denise C. Arruda, Jorge Augusto Borin Scutti, Alisson L. Matsuo, Carlos R. Figueiredo |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2013 |
| Předmět: |
Pyrostegia venusta
Programmed cell death biology Melanoma Pharmaceutical Science Apoptosis biology.organism_classification medicine.disease Molecular biology Acral lentiginous melanoma saturated hydrocarbons Biochemistry In vivo Drug Discovery parasitic diseases medicine melanoma DNA fragmentation Cytotoxic T cell cytotoxicity Original Article |
| Zdroj: | Pharmacognosy Magazine Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0973-1296 |
| Popis: | Made available in DSpace on 2015-03-18T15:56:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: Pyrostegia venusta (Ker. Gawl.) Miers (Bignoniacea) is a medicinal plant from the Brazilian Cerrado used to treat leucoderma and common diseases of the respiratory system. Objective: To investigate the antitumor activity of P.venusta extracts against melanoma. Materials and Methods: The cytotoxic activity and tumor induced cell death of heptane extract (HE) from P. venusta flowers was evaluated against murine melanoma B16F10-Nex2 cells in vitro and in a syngeneic model in vivo. Results: We found that HE induced apoptosis in melanoma cells by disruption of the mitochondrial membrane potential, induction of reactive oxygen species and late apoptosis evidenced by plasma membrane blebbing, cell shrinkage, chromatin condensation and DNA fragmentation, exposure of phosphatidylserine on the cell surface and activation of caspase-2,-3,-8,-9. HE was also protective against singeneyc subcutaneous melanoma HE compounds were also able to induce cell cycle arrest at G2/M phases on tumor cells. On fractionation of HE in silica gel we isolated a cytotoxic fraction that contained a mixture of saturated hydrocarbons identified by (1) H NMR and GC-MS analyses. Predominant species were octacosane (C 28 H 58 -36%) and triacontane (C 30 H 62 -13%), which individually showed significant cytotoxic activity against murine melanoma B16F10-Nex2 cells in vitro and a very promising antitumor protection against subcutaneous melanoma in vivo. Conclusion: The results suggest that the components of the heptane extract, mainly octasane and triacontane, which showed antitumor properties in experimental melanoma upon regional administration, might also be therapeutic in human cancer, such as in the mostly epidermal and slowly invasive melanomas, such as acral lentiginous melanoma, as an adjuvant treatment to surgical excision. Fed Univ Sao Paulo UNIFESP, Dept Microbiol Immunol & Parasitol, Div Cell Biol, BR-04023062 Sao Paulo, Brazil Fed Univ Sao Paulo UNIFESP, Expt Oncol Unit UNONEX, BR-04023062 Sao Paulo, Brazil Univ Estadual Paulista UNESP, Phytochem Lab, Dept Biol Sci, Assis, SP, Brazil Fed Univ Sao Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci, BR-04023062 Sao Paulo, Brazil Univ Estadual Paulista UNESP, Phytochem Lab, Dept Biol Sci, Assis, SP, Brazil |
| Databáze: | OpenAIRE |
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